N-glycan structures from the major glycoproteins of pigeon egg white -: Predominance of terminal Galα (1-4)Gal
N-glycan structures from the major glycoproteins of pigeon egg white -: Predominance of terminal Galα (1-4)Gal
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DOI：
10.1074/jbc.m101380200
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发表时间：
2001-06-29
影响因子：
4.8
通讯作者：
Lee, YC
中科院分区：
文献类型：
--
作者：
Takahashi, N;Khoo, KH;Lee, YC
酶促释放来自鸽子蛋白色的主要糖蛋白（卵转铁蛋白、卵类粘蛋白和卵清蛋白）的N-聚糖，并用2-氨基吡啶还原胺化，分离，并通过质谱和使用三种不同的高效液相色谱（HPLC）柱的三维映射技术进行结构表征（Takahashi，N.，中川，H.，Fujikawa，K.，Kawamura，Y.，和Tomiya，N.等（1995）Anal Biochem.226，139-146）。25个主要的N-聚糖结构，所有这些迄今未知的，被确定为吡啶基氨基衍生物。其中，13个是中性的，10个是单唾液酸，2个是二唾液酸寡糖。所有N-聚糖在非还原末端位置含有1 - 4个Gal（YC 1，4）GalP（1，4）序列，并且不含岩藻糖残基。N-乙酰神经氨酸仅与β-半乳糖α（2，6）-连接.来自四种不同糖蛋白的N-聚糖的HPLC图谱在定性上彼此非常相似，但峰分布不相同。单唾液酸聚糖在所有四种糖蛋白中含量最高，其次是中性聚糖，二唾液酸聚糖在卵转铁蛋白中含量最低，在卵类粘蛋白中含量最高。卵转铁蛋白中主要是具有二等分GlcNAc的三触角结构，其他糖蛋白中主要是四触角结构（具有和不具有二等分GlcNAc）。在所有四种糖蛋白中也发现了少量的五触角结构（具有唾液酸且没有二等分GlcNAc残基）。鸡蛋白色糖蛋白主要含有高甘露糖和杂合型，而鸽子蛋白色糖蛋白中的所有N-聚糖结构均为复杂型。
N-Glycans from major glycoproteins of pigeon egg white (ovotransferrin, ovomucoid, and ovalbumins) were enzymatically released and were reductively aminated with 2-aminopyridine, separated, and structurally characterized by mass spectrometry and a three-dimensional mapping technique using three different columns of high performance liquid chromatography (HPLC) (Takahashi, N., Nakagawa, H., Fujikawa, K., Kawamura, Y., and Tomiya,N. (1995) Anal Biochem. 226, 139-146). Twenty-five major N-glycan structures, all of them hitherto unknown, were identified as pyridylamino derivatives. Of these, 13 were neutral, 10 were monosialyl, and 2 were disialyl oligosaccharides. All N-glycans contain from one to four Gal(YC1,4)GalP(1,4) sequences at the nonreducing terminal positions and are devoid of fucose residues. NAcetylneuraminic acids were alpha (2,6)-linked only to beta -galactose. The HPLC profiles of the N-glycans from four different glycoproteins were qualitatively very similar to each other, but not identical in the peak distributions. Monosialyl glycans were most abundant in all four glycoproteins, followed by neutral glycans, Disialyl glycans were lowest in ovotransferrin, and highest in ovomucoid. Triantennary structures with bisecting GlcNAc were predominant in ovotransferrin, and tetra-antennary (with and without bisecting GlcNAc-containing) structures were predominant in other glycoproteins. Penta-antennary structures (with a sialic acid and without bisecting GlcNAc residue) were also found in small quantities in all four glycoproteins. In contrast to the chicken egg white counterparts, which contain mostly high mannose and hybrid types, all N-glycan structures in the major pigeon egg white glycoproteins are complex type.