Detection of chimerism following vascularized bone allotransplantation by polymerase chain reaction using a Y-chromosome specific primer
Detection of chimerism following vascularized bone allotransplantation by polymerase chain reaction using a Y-chromosome specific primer
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DOI：
10.1016/s0736-0266(03)00108-6
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发表时间：
2003-11-01
影响因子：
2.8
通讯作者：
Bishop, AT
中科院分区：
文献类型：
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作者：
Muramatsu, K;Valenzuela, RG;Bishop, AT
异基因器官移植后的嵌合现象是一种已知发生的现象，并与同种异体移植免疫耐受的发展有关。然而，很少有人知道移植细胞迁移后血管化骨同种异体移植。在这项研究中，嵌合体进行了评估后，从男性DA或PVG供体的血管化胫骨移植到女性PVG大鼠受体使用半定量聚合酶链反应的Y染色体。移植后给予FK-506（他克莫司）用于免疫抑制。移植后18周，所有免疫抑制的PVG大鼠接受PVG骨移植物的胸腺、脾脏、肝脏和颈部淋巴结中均显示出高水平的嵌合体（1%）。在对侧胫骨和肱骨中也检测到供体细胞。在非免疫抑制的PVG大鼠DA骨移植受体中，移植后2周内在5只大鼠中的3只中的脾脏中检测到供体细胞。在这些动物中，骨移植物被严重排斥。在DA骨移植的免疫抑制PVG大鼠受体中，在移植后1、12和18周，5只大鼠中的2只、8只大鼠中的4只和10只大鼠中的8只在外周血中显示低水平嵌合体（0.1%）。6只大鼠在脾脏和胸腺中表现出高水平的嵌合体。组织学研究显示，移植后18周内没有排斥反应。我们的研究结果表明，嵌合体，或存在于宿主组织中的移植细胞，可能会发生在面对急性排斥反应，并证明血管化同种异体和同种异体活骨移植后，当慢性免疫抑制维持。移植物血管在短期内的通畅性可能允许细胞迁移，即使是在面临急性排斥反应的情况下。移植物骨髓成分在宿主组织中的长期存活和增殖在适当的免疫抑制下是可能的。(C)2003年由Elsevier Ltd.代表骨科研究学会出版。
Chimerism following allogeneic organ transplantation is a phenomenon known to occur and be associated with development of immunologic tolerance in allotransplantation. However, little is known about graft cell migration following vascularized bone allografting. In this study, chimerism was assessed following vascularized tibia transplantation from male DA or PVG donors to female PVG rat recipients using a semi-quantitative polymerase chain reaction for the Y-chromosome. FK-506 (Tacrolimus) was administered after transplantation for immunosuppression. All immunosuppresssed PVG rat recipients of PVG bone grafts showed a high level of chimerism (1%) in the thymus, spleen, liver and cervical lymph nodes at 18 weeks post-transplant. Donor cells were also detected in the contralateral tibia and humerus. In non-immunosuppressed PVG rat recipients of DA bone grafts, donor cells were detected in the spleen in three of five rats within 2 weeks post-transplant. In these animals the bone grafts were severely rejected. In immunosuppressed PVG rat recipients of DA bone grafts, two of five, four of eight and eight of 10 rats showed low level chimerism (0.1%) in peripheral blood at 1, 12, and 18 weeks post-transplant. Six rats showed a high level of chimerism in the spleen and thymus. Histological studies revealed no rejection findings through 18 weeks post-transplant. Our results indicate that chimerism, or the presence of graft cells in host tissue, may occur in the face of acute rejection and be demonstrable following vascularized isograft and allograft living bone transplantation when chronic immunosuppression is maintained. Graft vascular patency during the short-term likely allows cellular migration, even in the face of acute rejection. Long-term survival and proliferation of graft marrow elements in host tissue may be possible with adequate immunosuppression. (C) 2003 Published by Elsevier Ltd. on behalf of Orthopaedic Research Society.