Clinical Utility of Insulin-Like Growth Factor 1 and 2; Determination by High Resolution Mass Spectrometry
Clinical Utility of Insulin-Like Growth Factor 1 and 2; Determination by High Resolution Mass Spectrometry
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DOI：
10.1371/journal.pone.0043457
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发表时间：
2012-09-11
期刊：
影响因子：
3.7
通讯作者：
Reitz, Richard
中科院分区：
文献类型：
--
作者：
Bystrom, Cory;Sheng, Shijun;Reitz, Richard
胰岛素样生长因子-1（IGF-I）的测量可用于生长障碍的诊断和管理，但由于大量的参考标准品、抗体、检测方法和分析前制备策略，结果的测定间比较变得复杂。我们开发了一种完整IGF-I的定量LC-MS方法，与依赖于胰蛋白酶肽稳定同位素稀释分析的质谱方法相比，该方法在通量和复杂性方面具有优势。由于该方法利用全扫描数据，该测定很容易扩展到使用相同的测定方案提供IGF-II的定量测量。经验证的IGF-I和IGF-II的LC-MS测定在儿科和成人参考范围内提供了准确的结果，适用于临床使用。
Measurement of insulin-like growth factor-1 (IGF-I) has utility for the diagnosis and management of growth disorders, but inter-assay comparison of results has been complicated by a multitude of reference standards, antibodies, detection methods, and pre-analytical preparation strategies. We developed a quantitative LC-MS method for intact IGF-I, which has advantages in throughput and complexity when compared to mass spectrometric approaches that rely on stable isotope dilution analysis of tryptic peptides. Since the method makes use of full-scan data, the assay was easily extended to provide quantitative measurement of IGF-II using the same assay protocol. The validated LC-MS assay for IGF-I and IGF-II provides accurate results across the pediatric and adult reference range and is suitable for clinical use.