Adaptation of the acetogen Clostridium thermoautotrophicum to minimal medium.
Adaptation of the acetogen Clostridium thermoautotrophicum to minimal medium.
复制标题
产乙酸菌热自养梭菌对基本培养基的适应。
DOI：
10.1128/jb.165.1.315-318.1986
复制
发表时间：
1986
影响因子：
3.2
通讯作者：
Drake,HL
中科院分区：
文献类型：
--
作者：
Savage,MD;Drake,HL
热自养梭菌适应于基本培养基，并以葡萄糖、甲醇或H2-CO2为代价进行培养。适应菌株的生长不需要补充氨基酸，烟酸是唯一的必需维生素。无论是N2还是硝酸盐都不能取代铵作为氮源，生物素优先刺激葡萄糖细胞系。在基本培养基中的生长产生了实质上更高的乙酸盐浓度每单位的生物量形成比不确定的培养基中的生长。
Clostridium thermoautotrophicum was adapted to minimal medium and cultivated at the expense of glucose, methanol, or H2-CO2. No supplemental amino acids were required for growth of the adapted strain, and nicotinic acid was the sole essential vitamin. Neither N2 nor nitrate could replace ammonium as the nitrogen source, and biotin was preferentially stimulatory for glucose cell lines. Growth in minimal medium yielded substantially higher acetate concentrations per unit of biomass formed than did growth in undefined medium.