Impact desolvation of electrosprayed microdroplets - a new ionization method for mass spectrometry of large biomolecules
Impact desolvation of electrosprayed microdroplets - a new ionization method for mass spectrometry of large biomolecules
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DOI：
10.1002/rcm.470
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发表时间：
2001-01-01
影响因子：
2
通讯作者：
Williams, P
中科院分区：
文献类型：
--
作者：
Aksyonov, SA;Williams, P
电喷雾微滴冲击去溶剂化是一种制备生物大分子气相离子的新方法。分析物溶解在电解质溶液中，该电解质溶液在真空中电喷雾，产生高度带电的微米和亚微米尺寸的液滴（微滴）。这些微滴通过类似于5 - 10 kV的电位差加速到几km/s的速度，并允许撞击目标表面。高能撞击使液滴蒸发并释放分析物分子的去溶剂化气相离子。寡核苷酸（2至12聚体）和肽（缓激肽，神经降压素）产生单电荷和双电荷分子离子，没有检测到碎片。由于多个充电的程度是显着小于在大气压电喷雾电离，和该方法产生的离子很大程度上不含加合物的高离子强度的解决方案，IDEM有一定的承诺作为一种方法耦合到液相色谱技术和混合物分析。离子在真空中在平坦的等电位表面产生，潜在地允许有效的离子提取。版权所有（C）2001约翰威利父子有限公司
Impact desolvation of electrosprayed microdroplets (IDEM) is a new method for producing gas-phase ions of large biomolecules. Analytes are dissolved in an electrolyte solution which is electrosprayed in vacuum, producing highly charged micron and sub-micron sized droplets (microdroplets). These microdroplets are accelerated through potential differences similar to5 - 10 kV to velocities of several km/s and allowed to impact a target surface. The energetic impacts vaporize the droplets and release desolvated gas-phase ions of the analyte molecules. Oligonucleotides (2- to 12-mer) and peptides (bradykinin, neurotensin) yield singly and doubly charged molecular ions with no detectable fragmentation. Because the extent of multiple charging is significantly less than in atmospheric pressure electrospray ionization, and the method produces ions largely free of adducts from solutions of high ionic strength, IDEM has some promise as a method for coupling to liquid chromatographic techniques and for mixture analysis. Ions are produced in vacuum at a flat equipotential surface, potentially allowing efficient ion extraction. Copyright (C) 2001 John Wiley & Sons, Ltd.