Trace analysis of per- and polyfluorinated alkyl substances (PFAS) in dried blood spots - Demonstration of reproducibility and comparability to venous blood samples.
Trace analysis of per- and polyfluorinated alkyl substances (PFAS) in dried blood spots - Demonstration of reproducibility and comparability to venous blood samples.
复制标题
干血斑中全氟烷基物质和多氟烷基物质 (PFAS) 的痕量分析 - 证明静脉血样本的再现性和可比性。
DOI：
10.1016/j.scitotenv.2023.163530
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
GodriPollitt,KrystalJ
中科院分区：
文献类型：
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作者：
Lin,ElizabethZ;Nason,SaraL;Zhong,Alexander;Fortner,John;GodriPollitt,KrystalJ
全氟烷基和多氟烷基物质（PFAS）是一组人造化学品，因其防污和防水性能而广泛用于消费品、个人护理和家用产品。PFAS暴露与各种不良健康结果有关。这种接触通常在静脉血样中进行评价。虽然这种样本类型可以从健康成年人中获得，但在评估脆弱人群时需要采用侵入性较小的采血方法。干血斑（DBS）由于相对容易收集、运输和储存，作为暴露评估的生物基质而受到关注。本研究的目的是开发和验证一种分析方法来测量DBS中的PFAS。提出了从DBS中提取PFAS的工作流程，通过液相色谱-高分辨率质谱法进行化学分析，对血液质量进行归一化，并进行空白校正以考虑潜在的污染。22种PFAS的回收率均在80%以上，平均变异系数为14%。DBS和6名健康成人配对全血样本中检测到的PFAS浓度的比较具有相关性（R2> 0.9）。研究结果表明，DBS中广泛范围的PFAS的痕量水平可以重复测量，并且与液体全血样本相当。DBS可以为环境暴露提供新的见解，包括在敏感性的关键窗口期间（即，在子宫内，早期生命），这在很大程度上是不确定的。
Per- and polyfluoroalkyl substances (PFAS) are a group of man-made chemicals that have been widely used in consumer, personal care, and household products for their stain- and water-repellent properties. PFAS exposure has been linked to various adverse health outcomes. Such exposure has commonly been evaluated in venous blood samples. While this sample type can be obtained from healthy adults, a less invasive method of blood collection is required when evaluating vulnerable populations. Dried blood spots (DBS) have gained attention as a biomatrix for exposure assessment given the relative ease of collection, transport, and storage. The objective of this study was to develop and validate an analytical method to measure PFAS in DBS. A workflow is presented for extracting PFAS from DBS, chemical analysis by liquid chromatography-high resolution mass spectrometry, normalization for blood mass, and blank correction to account for potential contamination. Over 80 % recovery was achieved for the 22 PFAS measured with an average coefficient of variation of 14 %. Comparison of PFAS concentrations detected in DBS and paired whole blood samples from six healthy adults was correlated (R2> 0.9). Findings demonstrate trace levels of a broad range of PFAS in DBS can be reproducibly measured and are comparable to liquid whole blood samples. DBS can offer novel insights to environmental exposures, including during critical windows of susceptibility (i.e., in utero, early life), which have been largely uncharacterized.