Tissue culture system for infection with human hepatitis delta virus.
Tissue culture system for infection with human hepatitis delta virus.
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用于感染人类丁型肝炎病毒的组织培养系统。
DOI：
10.1128/jvi.65.7.3443-3450.1991
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发表时间：
1991
影响因子：
5.4
通讯作者：
Lanford,RE
中科院分区：
文献类型：
--
作者：
Sureau,C;Jacob,JR;Eichberg,JW;Lanford,RE
开发了一种体外培养系统用于测定人类丁型肝炎病毒（HDV）的感染性。从黑猩猩肝脏中分离出肝细胞并在无血清培养基中生长。细胞可被 HDV 感染，并且在培养物中至少 3 周内仍对感染敏感，感染后 6 天就出现了 HDV 复制特征的 RNA 种类就证明了这一点。当对来自不含乙型肝炎病毒 (HBV) 的动物的细胞进行重复实验时，HDV 感染以一致的方式发生，但没有迹象表明接种物中存在 HBV 感染。尽管对不同来源的不含 HDV 的 HBV 接种物进行了多次尝试，但没有证据表明这些细胞对 HBV 感染具有敏感性。这一观察结果可能表明乙型肝炎病毒和丁型肝炎病毒使用不同的方式进入肝细胞。当源自 HBV 感染动物的细胞暴露于 HDV 时，除了 HBV 复制和 Dane 颗粒的产生外，还获得了子代 HDV 颗粒的合成和释放。虽然黑猩猩肝细胞不感染乙型肝炎病毒，但其原代培养物能够支持乙型肝炎病毒的部分生命周期和丁型肝炎病毒的整个生命周期。
An in vitro culture system was developed for assaying the infectivity of the human hepatitis delta virus (HDV). Hepatocytes were isolated from chimpanzee liver and grown in a serum-free medium. Cells were shown to be infectible by HDV and to remain susceptible to infection for at least 3 weeks in culture, as evidenced by the appearance of RNA species characteristic of HDV replication as early as 6 days postinfection. When repeated experiments were carried out on cells derived from an animal free of hepatitis B virus (HBV), HDV infection occurred in a consistent fashion but there was no indication of infection with the HBV that was present in the inoculum. Despite numerous attempts with different sources of HBV inocula free of HDV, there was no evidence that indicated susceptibility of these cells to HBV infection. This observation may indicate that HBV and HDV use different modes of entry into hepatocytes. When cells derived from an HBV-infected animal were exposed to HDV, synthesis and release of progeny HDV particles were obtained in addition to HBV replication and production of Dane particles. Although not infectible with HBV, primary cultures of chimpanzee hepatocytes are capable of supporting part of the life cycle of HBV and the entire life cycle of HDV.