CIR AND FIU PROTEINS IN THE OUTER-MEMBRANE OF ESCHERICHIA-COLI CATALYZE TRANSPORT OF MONOMERIC CATECHOLS - STUDY WITH BETA-LACTAM ANTIBIOTICS CONTAINING CATECHOL AND ANALOGOUS GROUPS
CIR AND FIU PROTEINS IN THE OUTER-MEMBRANE OF ESCHERICHIA-COLI CATALYZE TRANSPORT OF MONOMERIC CATECHOLS - STUDY WITH BETA-LACTAM ANTIBIOTICS CONTAINING CATECHOL AND ANALOGOUS GROUPS
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DOI：
10.1128/jb.172.3.1361-1367.1990
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发表时间：
1990-03-01
影响因子：
3.2
通讯作者：
ROSENBERG, EY
中科院分区：
文献类型：
--
作者：
NIKAIDO, H;ROSENBERG, EY
最近，β-已经开发了含有铁螯合部分的内酰胺试剂，例如含有邻苯二酚的E0702和含有3-羟基吡啶酮的吡唑莫南和U-78，608。通过确定缺乏各种铁抑制性外膜蛋白的大肠杆菌突变体对这些试剂的敏感性，我们发现这些蛋白质中的两个具有迄今为止未知的功能，Fiu和Cir，显然参与了单体儿茶酚及其类似物的运输。这些结果证实了Curtis及其同事的结论，该结论是通过使用一组不同的含儿茶酚抗生素（N。A. C.柯蒂斯河L.艾森施塔特，S.伊斯特河康福德湖A.步行者和A. J.白色，抗微生物剂。探员Chemother 32：1879-1886，1988）。E0702被证明可以增强放射性三价铁在野生型细胞中的摄取，但不能增强cir fiu双突变体的摄取。通过将E0702的流入与其通过周质β-葡聚糖酶的水解相结合，通过对E0702-Fe 3+复合物的转运，我们发现野生型细胞转运未配体的E0702的速率与E0702-Fe 3+复合物的转运速率相当或甚至更高。我们推测，Cir和Fiu的主要功能可能是重新捕获肠杆菌素的水解产物，如2，3-二羟基苯甲酸和2，3-二羟基苯甲酰丝氨酸。
Recently, .beta.-lactam agents containing iron-chelating moieties, such as E0702, which contains catechol, and pirazmonam and U-78,608, which contain 3-hydroxypyridone, have been developed. By determining the susceptibility to these agents of Escherichia coli mutants lacking various iron-repressible outer membrane proteins, we showed that two of these proteins with hitherto unknown functions, Fiu and Cir, were apparently involved in the transport of monomeric catechol and its analogs. These results confirm the conclusion of Curtis and co-workers, which was obtained by using a different set of catechol-containing antibiotics (N. A. C. Curtis, R. L. Eisenstadt, S. J. East, R. J. Cornford, L. A. Walker, and A. J. White, Antimicrob. Agents Chemother. 32:1879-1886, 1988). E0702 was shown to enhance the uptake of radioactive ferric iron into wild-type cells but not into cir fiu double mutants. By combining the influx of E0702 with its hydrolysis by a periplasmic .beta.-lactamase, we showed that the wild-type cells transported unliganded E0702 at a rate comparable to or even higher than the rate of transport of the E0702-Fe3+ complex. We postulate that the main function of Cir and Fiu may be to recapture the hydrolytic products of enterobactin, such as 2,3-dihydroxybenzoic acid and 2,3-dihydroxybenzoylserine.