A low-sample-loss microfluidic system for the quantification of size-independent cellular electrical property Its demonstration for the identification and characterization of circulating tumour cells (CTCs)
A low-sample-loss microfluidic system for the quantification of size-independent cellular electrical property Its demonstration for the identification and characterization of circulating tumour cells (CTCs)
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用于量化与尺寸无关的细胞电特性的低样品损失微流体系统 - 循环肿瘤细胞 (CTC) 识别和表征的演示
DOI：
10.1016/j.snb.2017.02.048
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发表时间：
2017-07-01
影响因子：
8.4
通讯作者：
Wu, Min-Hsien
中科院分区：
文献类型：
--
作者：
Chiu, Tzu-Keng;Zhao, Yang;Wu, Min-Hsien
目前表征细胞电特性的方法通常需要具有高细胞数量的测试样品，这限制了它们在样品中细胞数量有限的情况下的应用。为了解决这个问题，这项研究提出了一种低样品损失的微流体系统，能够表征与尺寸无关的电特性（例如，比膜电容和细胞质电导率）。在该设计中，使用毛细管将细胞直接转移到微流体收缩微通道的入口中，以尽可能减少细胞样品损失。结果表明，微流控系统能够显著减少样品损失现象，使细胞处理率由原来的0.2%提高到49.3- 60.0%。此外，作为证明，实验评估了使用所提出的方法在CTC分离过程后鉴定EpCAM阳性CTC以及区分三种不同（肝癌、口腔癌和肺癌）癌症类型的EpCAM阳性CTC的可行性。在探索的实验条件下，我们的研究结果表明，所提出的方法能够显著地从周围的EpCAM阴性细胞中识别EpCAM阳性CTC，以及基于其独特的细胞电特性来区分所测试的三种不同癌症类型的EpCAM阳性CTC。作为一个整体，提出的微流控系统被发现有用的CFC的识别后，传统的CTC isolatiOn过程。除了CTC鉴定外，所提出的方法可能具有检测细胞量有限的细胞悬液样品中特定细胞种类的潜力。(C)2017爱思唯尔B. V.保留所有权利。
The current approaches for the characterization of cellular electrical properties normally require a tested sample with high cell quantity, restricting their application in the cases in which the cell number in a sample is limited. To address this issue, this study presented a low-sample-loss microfluidic system capable of characterizing the size-independent electrical properties (e.g., specific membrane capacitance and cytoplasm conductivity) of single cells. In the design, a capillary tube was used to transfer cells directly into the entrance of a microfluidic constriction microchannel to possibly minimize cell sample loss. Results revealed that the microfluidic system was able to significantly reduce the sample loss phenomenon, by which the cell processing ratio could be raised from the original 0.2% to 49.3-60.0%. As a demonstration, moreover, the feasibility of using the proposed method for the identification of the EpCAM-positive CTCs after a CTC isolation process, and for the differentiation of the EpCAM-positive CTCs of three different (hepatic, oral and lung) cancer types were experimentally evaluated. Within the experimental conditions explored, our findings indicated that the proposed method was able to significantly identify the EpCAM-positive CTCs from the surrounding EpCAM-negative cells, as well as to differentiate the EpCAM-positive CTCs of the three different cancer types tested, based on their unique electrical properties of cells. As a whole, the proposed microfluidic system was found useful for the identification of CFCs after a conventional CTC isolatiOn process. In addition to CTC identification, the proposed method might hold potential for the detection of a specific cell species in a cell suspension sample with limited cell quantity. (C) 2017 Elsevier B.V. All rights reserved.