MicroRNA-203 suppresses cell proliferation and migration by targeting BIRC5 and LASP1 in human triple-negative breast cancer cells.
MicroRNA-203 suppresses cell proliferation and migration by targeting BIRC5 and LASP1 in human triple-negative breast cancer cells.
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DOI：
10.1186/1756-9966-31-58
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发表时间：
2012-06-19
期刊：
影响因子：
--
通讯作者：
Shi Y
中科院分区：
文献类型：
--
作者：
Wang C;Zheng X;Shen C;Shi Y
本研究旨在研究microRNA-203（miR-203）对三阴性乳腺癌（TNBC）细胞增殖和迁移的影响。实时荧光定量PCR检测miR-203在TNBC细胞系中的表达。将miR-203前体和对照microRNA（miRNA）转染到三阴性乳腺癌（TNBC）细胞系中，研究miR-203上调对细胞增殖和迁移的影响。同时检测了杆状病毒IAP重复序列蛋白5（BIRC 5）和Lim和SH 3结构域蛋白1（LASP 1）的mRNA和蛋白水平。还进行荧光素酶测定以验证BIRC 5和LASP 1作为miR-203靶标。miR-203和BIRC 5 siRNA均显著抑制TNBC细胞中的细胞增殖。miR-203和LASP 1 siRNA均显著抑制TNBC细胞中的细胞迁移。此外，BIRC 5和LASP 1的上调能够消除由miR-203前体转染诱导的作用。这些数据表明，miR-203可能在TNBC细胞中起肿瘤抑制剂的作用。因此，miR-203可能是该疾病的潜在治疗靶点。
This study was performed to investigate the effect of microRNA-203 (miR-203) on cell proliferation and migration in triple-negative breast cancer (TNBC). Real-time PCR was performed to detect the expression of miR-203 in TNBC cell lines. miR-203 precursor and control microRNA (miRNA) were transfected into triple-negative breast cancer (TNBC) cell lines and the effects of miR-203 up-regulation on the proliferation and migration of cells were investigated. Meanwhile, the mRNA and protein levels of baculoviral IAP repeat-containing protein 5 (BIRC5) and Lim and SH3 domain protein 1 (LASP1) were measured. Luciferase assays were also performed to validate BIRC5 and LASP1 as miR-203 targets. Both miR-203 and BIRC5 siRNA signicantly inhibited cell proliferation in TNBC cells. Both miR-203 and LASP1 siRNA signicantly inhibited cell migration in TNBC cells, also. Moreover, up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor. These data suggest that miR-203 may function as a tumor suppressor in TNBC cells. Thus, miR-203 could be a potential therapeutic target for this disease.
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