Identification of eight genes encoding chemokine-like factor superfamily members 1-8 (CKLFSF1-8) by in silico cloning and experimental validation
Identification of eight genes encoding chemokine-like factor superfamily members 1-8 (CKLFSF1-8) by in silico cloning and experimental validation
复制标题
DOI：
10.1016/s0888-7543(03)00095-8
复制
发表时间：
2003-06-01
期刊：
影响因子：
4.4
通讯作者：
Ma, DL
中科院分区：
文献类型：
--
作者：
Han, WL;Ding, PG;Ma, DL
TM 4SF 11与染色体16 q13上由SCYA 22、SCYD 1和SCYA 17组成的趋化因子基因簇相距仅102 kb。CKLF定位于染色体16 q22。CKLF具有与CCL 22/MDC、CX 3CL 1/fractalkine、CCL 17/TARC和TM 4SF蛋白相关的一些特征。基于CKLF 2 cDNA和蛋白质序列的生物信息学结合实验验证鉴定了8个新基因，命名为趋化因子样因子超家族成员1-8（CKLFSF 1 -8）。CKLFSF 1 -8形成基因簇，CKLF 2和CKLFSF 1 -8之间的序列同源性为12.5%至39.7%。大多数CKLFSF具有选择性RNA剪接形式。CKLFSF 1具有CC基序，与趋化因子的序列相似性高于任何其他CKLFSF。CKLFSF 8与TM 4SF 11具有39.3%的氨基酸同一性。CKLFSF 1将CKLFSF家族与趋化因子联系起来，CKLFSF 8将其与TM 4SF联系起来。CKLFSF 2 -7的特性介于CKLFSF 1和CKLFSF 8之间。这表明CKLFSF代表SCY和TM 4SF基因家族之间的新基因家族。(C)2003 Elsevier Science（美国）。All rights reserved.
TM4SF11 is only 102 kb from the chemokine gene cluster composed of SCYA22, SCYD1, and SCYA17 on chromosome 16q13. CKLF maps on chromosome 16q22. CKLFs have some characteristics associated with the CCL22/MDC, CX3CL1/fractalkine, CCL17/TARC, and TM4SF proteins. Bioinformatics based on CKLF2 cDNA and protein sequences in combination with experimental validation identified eight novel genes designated chemokine-like factor superfamily members 1-8 (CKLFSF1-8). CKLFSF1-8 form gene clusters; the sequence identities between CKLF2 and CKLFSF1-8 are from 12.5 to 39.7%. Most of the CKLFSFs have alternative RNA splicing forms. CKLFSF1 has a CC motif and higher sequence similarity with chemokines than with any of the other CKLFSFs. CKLFSF8 shares 39.3% amino acid identity with TM4SF11. CKLFSF1 links the CKLFSF family with chemokines, and CKLFSF8 links it with TM4SF. The characteristics of CKLFSF2-7 are intermediate between CKLFSF1 and CKLFSF8. This indicates that CKLFSF represents a novel gene family between the SCY and the TM4SF gene families. (C) 2003 Elsevier Science (USA). All rights reserved.