A putative integrase gene defines the distal end of a large cluster of ToxR-regulated colonization genes in Vibrio cholerae
A putative integrase gene defines the distal end of a large cluster of ToxR-regulated colonization genes in Vibrio cholerae
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DOI：
10.1099/13500872-142-8-2165
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发表时间：
1996-08-01
期刊：
影响因子：
2.8
通讯作者：
Peterson, KM
中科院分区：
文献类型：
--
作者：
Kovach, ME;Shaffer, MD;Peterson, KM
编码参与霍乱弧菌辅助定植因子 (ACF) 表达和毒素共调节菌毛 (TCP) 生物发生的一大簇毒力基因的两侧是类似于噬菌体附着 (att) 半位点的序列。与 attL 样位点相邻的是一个基因 (int)，它编码与位点特异性重组酶整合酶家族相关的蛋白质。假定的弧菌整合酶似乎与大肠杆菌隐性原噬菌体 (CP4-57) 整合酶蛋白关系最密切（52% 同一性，73% 相似性）。对多种霍乱弧菌菌株（01、非01和0139）的基因组分析表明，只有能够引起流行性亚洲霍乱的弧菌才拥有与整合酶相关的TCP-ACF定植基因簇。无毒菌株中整合酶基因缺失的事实表明霍乱弧菌流行菌株通过水平转移获得了TCP-ACF定植基因簇。
A large cluster of virulence genes encoding proteins involved in Vibrio cholerae accessory colonization factor (ACF) expression and toxin-coregulated pilus (TCP) biogenesis is flanked by sequences that resemble bacteriophage attachment (att) half-sites. Adjacent to the attL-like site is a gene (int) that encodes a protein related to the integrase family of site-specific recombinases. The putative vibrio integrase appears to be most closely related to the Escherichia coli cryptic prophage (CP4-57) integrase protein (52% identity, 73% similarity). Genomic analysis of numerous V. cholerae strains (01, non-01 and 0139) revealed that only vibrios capable of causing epidemic Asiatic cholera possess the TCP-ACF colonization gene cluster in association with the integrase. The fact that the integrase gene is absent in avirulent strains suggests that epidemic strains of V. cholerae obtained the TCP-ACF colonization gene cluster via horizontal transfer.