Rapid Sex-typing of Asparagus for Male Hybrid Seed Production using n-Propyl N-(3,4-dichlorophenyl)carbamate (NPC)
Rapid Sex-typing of Asparagus for Male Hybrid Seed Production using n-Propyl N-(3,4-dichlorophenyl)carbamate (NPC)
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使用 N-(3,4-二氯苯基)氨基甲酸正丙酯 (NPC) 快速对芦笋进行性别分型，用于雄性杂交种子生产
DOI：
10.21273/hortsci.34.6.1090
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发表时间：
1999
期刊：
影响因子：
1.9
通讯作者：
E. Durner
中科院分区：
文献类型：
--
作者：
M. Aneja;T. Gianfagna;S. Garrison;E. Durner
芦笋可诱导早熟开花。使用N-苯基氨基甲酸酯类除草剂(如N-丙基-N-(3，4-二氯苯基)氨基甲酸酯)处理的种子中，只有50%的种子产生开花植株，因为这些化合物抑制发芽和出苗。我们通过确定环境条件、时间、剂量和持续时间来改进处理方法，以最大限度地提高萌发率、出苗率和开花率。种子在水中浸泡5d，发芽种子在胚根萌发后用0.4mM NPC处理5d，幼苗在光照下通风，90%的处理种子产生开花幼苗。对于新收获的种子，发芽率的变化比老化的种子更大，单株幼苗必须在胚根出现后1天内转移到NPC，才能产生高比例的开花植株。在7个芦笋雄性品种中，用NPC化学诱导幼苗开花产生了与大田植株相似的性别比，表明NPC诱导开花不改变花的分化或性别表达。该方法可用于快速、准确地鉴定“雄性”品种的雌株比例。
Precocious flowering can be induced in asparagus ( Asparagus officinalis L.) seedlings with N-phenylcarbamate herbicides, such as n-propyl N-(3,4-dichlorophenyl) carbamate (NPC); however, only ≈50% of the treated seeds produce flowering plants because these compounds inhibit germination and seedling emergence. We have improved the treatment method by determining the environmental conditions, timing, dose, and duration needed to maximize the percentage of germination, emergence, and flowering. Imbibing seeds in water for 5 days, and then treating germinated seeds with 0.4 m M NPC for 5 days after radicle emergence, with seedling aeration in the light, resulted in the production of flowering seedlings from >90% of the treated seeds. For freshly harvested seeds, in which germination rates are more variable than aged seeds, individual seedlings must be transferred to NPC within 1 day after radicle emergence to produce a high percentage of flowering plants. For seven male asparagus cultivars, chemical induction of flowering in seedlings with NPC produced a sex ratio similar to that of field-grown plants, demonstrating that NPC induces flowering without altering floral differentiation or sex expression. This method can be used for rapidly and accurately identifying the percentage of females in "male" cultivars.