Temporal and spatial patterns of osteoblast activation following implantation of beta-TCP particles into bone.
Temporal and spatial patterns of osteoblast activation following implantation of beta-TCP particles into bone.
复制标题
将 β-TCP 颗粒植入骨后成骨细胞激活的时间和空间模式。
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
U. Gross
中科院分区：
文献类型：
--
作者：
M. Neo;H. Herbst;C. Voigt;U. Gross
通过地高辛标记的前胶原α 1（I）RNA探针原位杂交分析植入骨中的β-TCP颗粒周围成骨细胞活化的时间和空间模式。将β-TCP颗粒（直径150-300微米）植入大鼠胫骨，并在术后3、5、7、14和28天收集标本。活化的成骨细胞显示强烈的前胶原α 1（I）RNA特异性标记。在第3天，成骨细胞内衬预先存在的骨小梁的地方显示出特定的信号。此外，在填充β-TCP颗粒之间空间的红细胞中的小梁附近也观察到与前成骨细胞相容的分散的活化细胞。很少观察到β-TCP表面的成骨细胞活化。在第5天和第7天，成骨细胞活化和骨形成向中心推进。在骨形成的最前沿，阳性细胞散在分布于血细胞凝块中，部分阳性细胞定植形成新的骨基质。新骨的形成并不总是开始于β-TCP的表面。在第14天，大多数β-TCP颗粒与新形成的骨紧密结合，阳性成骨细胞的数量减少。在第28天，零星地显示多核细胞对新形成的骨和β-TCP的吸收。这些细胞通常伴随着活跃的成骨细胞，表明早期骨重建。总之，原位杂交与前胶原α 1（I），以证明精确的骨细胞前体的招聘模式。β-TCP并不正向引导表达胶原I的骨细胞沿其表面沿着流动。对骨再生无明显影响。
Temporal and spatial patterns of osteoblast activation around beta-TCP particles implanted into bone were analyzed by in situ hybridization with digoxygenin-labeled procollagen alpha 1(I) RNA probes. beta-TCP particles (150-300 microns in diameter) were implanted into rat tibiae, and specimens were collected 3, 5, 7, 14, and 28 days after operation. Activated osteoblasts displayed intense procollagen alpha 1(I) RNA specific labeling. At day 3, osteoblasts lining pre-existing trabeculae in places showed a specific signal. Additionally, scattered activated cells compatible with preosteoblasts also were observed in the vicinity of the trabeculae among red blood cells that filled the space between beta-TCP particles. Osteoblast activation on the surface of beta-TCP rarely was observed. At days 5 and 7, osteoblast activation and bone formation advanced centripetally. At the forefront of bone formation positive cells were scattered in the blood cell clots, and some of the positive cells colonized forming new bone matrix. Formation of new bone did not always begin at the surface of beta-TCP. At day 14, most of the beta-TCP particles were tightly associated with newly formed bone, and the number of positive osteoblasts was reduced. At day 28, absorption of the newly formed bone and the beta-TCP by multinuclear cells was sporadically demonstrated. Such cells often were accompanied by active osteoblasts, suggesting early bone remodeling. In conclusion, in situ hybridization with procollagen alpha 1(I) was employed to demonstrate precisely the mode of recruitment of bone cell precursors. beta-TCP does not positively guide collagen I expressing bone cells along its surface. It has no apparent effects on bone regeneration.
影响因子：
2.9
作者：
GENOVESE, C;ROWE, D;KREAM, B
通讯作者：
KREAM, B