Solvent effects on the screening of parallel combinatorial libraries for selectors for chiral chromatography.
Solvent effects on the screening of parallel combinatorial libraries for selectors for chiral chromatography.
复制标题
溶剂对手性色谱选择器平行组合文库筛选的影响。
DOI：
10.1093/chromsci/40.7.383
复制
发表时间：
2002
影响因子：
1.3
通讯作者：
Li,Tingyu
中科院分区：
文献类型：
--
作者：
Gehring,AndrewP;Bluhm,LouisH;Ma,Yufeng;Li,Tingyu
研究了树脂溶胀特性与我们的平行文库筛选方法中使用的关键平衡测定结果之间的相关性。研究发现，将 CHCl3（聚苯乙烯和 TentaGel 树脂的有效溶胀溶剂）加入到平衡溶剂中可以加快平衡速度，从而缩短文库筛选时间。还发现平衡实验的结果取决于固体基础树脂的化学性质。看起来，具有相对惰性表面的聚苯乙烯树脂比极性 TentaGel 树脂提供了更高的对映选择性。充分溶胀的树脂对于直接测定树脂上的官能团的重要性得到了证明。
The correlation between the resin-swelling property and the outcome of the crucial equilibration assay used in our parallel library screening method is investigated. It is found that the incorporation of CHCl3(an effective swelling solvent for both the polystyrene and TentaGel resins) into the equilibration solvent leads to faster equilibration and thus shorter library screening time. The outcome of the equilibration experiment is also found to depend on the chemical nature of the solid base resins. It appears that polystyrene resin, which has a relatively inert surface, provides higher enantioselectivity than the polar TentaGel resin. The importance of a thoroughly swelled resin for the direct assay of functional groups on the resin is demonstrated.