Wnt signaling and a Hox protein cooperatively regulate PSA-3/Meis to determine daughter cell fate after asymmetric cell division in C-elegans
Wnt signaling and a Hox protein cooperatively regulate PSA-3/Meis to determine daughter cell fate after asymmetric cell division in C-elegans
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DOI：
10.1016/j.devcel.2006.04.020
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发表时间：
2006-07-01
影响因子：
11.8
通讯作者：
Sawa, Hitoshi
中科院分区：
文献类型：
--
作者：
Arata, Yukinobu;Kouike, Hiroko;Sawa, Hitoshi
不对称细胞分裂是实现细胞多样性的机制。在秀丽隐杆线虫中，许多不对称细胞分裂是由 Wnt-MAPK 通路通过 POP-1/TCF 控制的。然而，人们对 POP-1 如何决定子细胞的具体命运知之甚少。我们发现 nob-1/Hox、ceh-20/Pbx 和 Meis 相关基因 psa-3 是皮下 T 细胞不对称分裂所必需的。 psa-3 表达在 T 细胞子代之间不对称，并且它通过 psa-3 基因中的 POP-1 结合位点受 POP-1 调节。 psa-3 表达也通过 psa-3 内含子中的 NOB-1 结合序列受到 NOB-1 和 CEH-20 的调节。 PSA-3可以结合CEH-20并在T细胞分裂后发挥作用，促进子细胞的正确命运。这些结果表明 Wnt 信号传导和 Hox 蛋白之间的合作可决定子细胞的特定命运。
Asymmetric cell division is a mechanism for achieving cellular diversity. In C. elegans, many asymmetric cell divisions are controlled by the Wnt-MAPK pathway through POP-1/TCF. It is poorly understood, however, how POP-1 determines the specific fates of daughter cells. We found that nob-1/Hox, ceh-20/Pbx, and a Meis-related gene, psa-3, are required for asymmetric division of the T hypodermal cell. psa-3 expression was asymmetric between the T cell daughters, and it was regulated by POP-1 through a POP-1 binding site in the psa-3 gene. psa-3 expression was also regulated by NOB-1 and CEH-20 through a NOB-1 binding sequence in a psa-3 intron. PSA-3 can bind CEH-20 and function after the T cell division to promote the proper fate of the daughter cell. These results indicate that cooperation between Wnt signaling and a Hox protein functions to determine the specific fate of a daughter cell.