In vivo dynamics of human cord blood-derived CD34- SCID-repopulating cells using intra-bone marrow injection
In vivo dynamics of human cord blood-derived CD34- SCID-repopulating cells using intra-bone marrow injection
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DOI：
10.1038/leu.2009.206
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发表时间：
2010-01-01
期刊：
影响因子：
11.4
通讯作者：
Sonoda, Y.
中科院分区：
文献类型：
--
作者：
Kimura, T.;Matsuoka, Y.;Sonoda, Y.
人类CD34阴性（CD34(-)）造血干细胞（HSC）的鉴定为人类HSC区室的等级划分提供了新的概念。本研究研究了人脐带血来源的CD34（-）严重联合免疫缺陷(SCID)-再填充细胞（src）的长期再填充能力和再分配动力学，并使用骨髓内注射（IBMI）将其与CD34(+)CD38（+）和CD34(+)CD38(-) src进行比较，以阐明CD34(-) src的特征。在有限稀释分析数据的基础上，通过IBMI将估计数量的CD34(+)CD38（+）、CD34(+)CD38（-）和CD34(-) src移植到NOD/SCID小鼠。连续观察了注射部位和其他骨骼的人类细胞再生情况。有趣的是，CD34(+)CD38（+）、CD34(+)CD38（-）和CD34(-) src分别在移植后2周和5周开始向其他骨骼迁移。因此，CD34（+）和CD34(-) src的迁移起始似乎有所不同。此外，CD34(+)CD38(+) src只能维持短期的再繁殖。然而，CD34（+）、CD38（-）和CD34(-) src都具有较长期的再生能力。综上所述，这些发现表明CD34(-) src在体内表现出不同的动力学，从而表明与CD34(+)CD38（-）和CD34(+)CD38(-) src相比，鉴定的CD34(-) src是一类不同的原始hsc。白血病（2010）24,162-168；doi: 10.1038 / leu.2009.206;2009年10月1日在线发布
The identification of human CD34-negative (CD34(-)) hematopoietic stem cells (HSCs) provides a new concept for the hierarchy in the human HSC compartment. This study investigated the long-term repopulating capacity and redistribution kinetics of human cord blood-derived CD34(-) severe combined immunodeficiency (SCID)-repopulating cells (SRCs) and compared them with those of CD34(+)CD38(+) and CD34(+)CD38(-) SRCs using the intra-bone marrow injection (IBMI) to clarify the characteristics of CD34(-) SRCs. On the basis of the limiting dilution analyses data, estimated numbers of CD34(+)CD38(+), CD34(+)CD38(-), and CD34(-) SRCs were transplanted to NOD/SCID mice by IBMI. The human cell repopulation at the site of injection and the other bones were serially investigated. Interestingly, CD34(+)CD38(+), CD34(+)CD38(-), and CD34(-) SRCs began to migrate to other bones 2 and 5 weeks after the transplantation, respectively. Accordingly, the initiation of migration seemed to differ between the CD34(+) and CD34(-) SRCs. In addition, CD34(+)CD38(+) SRCs only sustained a short-term repopulation. However, both CD34(+)CD38(-) and CD34(-) SRCs had longer-term repopulation capacity. Taken together, these findings showed that CD34(-) SRCs show different in vivo kinetics, thus suggesting that the identified CD34(-) SRCs are a distinct class of primitive HSCs in comparison to the CD34(+)CD38(-) and CD34(+)CD38(-) SRCs. Leukemia (2010) 24, 162-168; doi:10.1038/leu.2009.206; published online 1 October 2009