IL-4-producing murine T helper cell line provides help for in vitro production of IgE.
IL-4-producing murine T helper cell line provides help for in vitro production of IgE.
复制标题
产生 IL-4 的鼠 T 辅助细胞系为 IgE 的体外生产提供帮助。
DOI：
10.1159/000235474
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Ishizaka,K
中科院分区：
文献类型：
--
作者：
Poulsen,LK;Katamura,K;Ishizaka,K
由于在体外使淋巴细胞产生IgE的困难，对IgE合成调节的研究受到阻碍。IgE，IgG 1和IgM的合成进行了研究，在小鼠共培养系统中，其中一个Conalbumin特异性的辅助性T细胞系的Th 2型一起培养与非贴壁的脾细胞从正常的MHC-匹配的小鼠。由抗原刺激的T细胞系提供的帮助诱导了显著的IgE产生（20 ng/ml），沿着IgG 1（5 μg/ml）和IgM（250 μg/ml）。培养物中的免疫球蛋白合成在第3-4天可检测到，并在第7-8天达到顶峰。在培养的前2天内观察到IL-4的产生。三种同种型的合成动力学是平行的。用Con A刺激Th 2细胞系产生类似于抗原刺激后的剂量-反应曲线，而来自这些细胞的上清液不能诱导IgE的合成。干扰素-γ在培养物中的存在抑制IgE、IgM和IgG 1的合成，但这种抑制不是由于干扰IL-4的产生，IL-4的产生在高剂量干扰素-γ的存在下增加。
Studies of the regulation of the IgE synthesis have been hampered by the difficulties in making lymphocytes produce IgE in vitro. Synthesis of IgE, IgG1 and IgM were investigated in a murine coculture system in which a conalbumin-specific T helper cell line of Th2 type was cultured together with nonadherent splenocytes from normal MHC-matched mice. Help provided by the antigen-stimulated T cell line induced significant IgE production (20 ng/ml), along with IgG1 (5 μg/ml) and IgM (250 μg/ml). Immunoglobulin synthesis in cultures was detectable at day 3–4 and culminated at day 7–8. IL-4 production was observed within the first 2 days of culture. The kinetics of the synthesis of the three isotypes were parallel. Stimulation of the Th2 cell line with Con A produced dose-response curves resembling those after antigen stimulation, whereas supernatant from these cells was unable to induce synthesis of IgE. The presence of interferon-γ in the cultures, inhibited synthesis of IgE, IgM and IgG1, but this inhibition was not due to interference with the IL-4 production, which increased in the presence of high doses of interferon-γ.