Chicken B lymphoma DT40 cells as a useful tool for in vitro analysis of pathogenic infectious bursal disease virus
Chicken B lymphoma DT40 cells as a useful tool for in vitro analysis of pathogenic infectious bursal disease virus
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DOI：
10.1292/jvms.70.407
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发表时间：
2008-04-01
影响因子：
1.2
通讯作者：
Fukushi, Hideto
中科院分区：
文献类型：
--
作者：
Terasaki, Kaori;Hirayama, Haruko;Fukushi, Hideto
研究了 DT40 细胞对传染性法氏囊病病毒 (IBDV) 致病野株（包括强毒株和经典毒株）的敏感性。病毒在DT40细胞中第一次和第二次传代后，在接种了致病性野毒株感染的法氏囊匀浆的DT40细胞中很容易检测到IBDV特异性抗原。 VP2 高变区的核苷酸序列分析对 IBDV 的毒力至关重要，结果表明，根据 DT40 细胞中的繁殖情况，致病性 IBDV 之间没有常见的氨基酸取代。这些结果表明 DT40 细胞是快速分离致病性野外菌株和连续体外分析 IBDV 的有用工具。
Susceptibility of DT40 cells to pathogenic field strains of infectious bursal disease virus (IBDV) including very virulent and classical virulent strains were studied. After the first and second passage of the virus in DT40 cells, IBDV-specific antigen was readily detected in DT40 cells inoculated with the pathogenic field strain infected bursal homogenates. Nucleotide sequence analysis in the VP2 hypervariable domain, which is critical for the virulence of IBDV, revealed no common amino acid substitutions among the pathogenic IBDVs in accordance with the propagation in DT40 cells. These results indicate that DT40 cells are a useful tool for rapid isolation of pathogenic field strains and successive in vitro analysis of IBDV.