A EUKARYOTIC TRANSCRIPTIONAL REPRESSOR WITH CARBOXYPEPTIDASE ACTIVITY
A EUKARYOTIC TRANSCRIPTIONAL REPRESSOR WITH CARBOXYPEPTIDASE ACTIVITY
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DOI：
10.1038/378092a0
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发表时间：
1995-11-02
期刊：
影响因子：
64.8
通讯作者：
RO, HS
中科院分区：
文献类型：
--
作者：
HE, GP;MUISE, A;RO, HS
脂肪细胞分化涉及甘油三酯代谢中几个基因的转录激活，包括编码脂肪细胞脂质结合蛋白ALBP的脂肪P2（aP 2或422）基因（1-3）。在小鼠aP 2启动子区域内，AE 1序列在aP 2基因表达的调节中作为阳性或阴性元件发挥作用（4-8）。AE-1序列是阳性鼠（3 T3）脂肪细胞因子C/EBP-α（5，6）、几种人前脂肪细胞因子（7）和一种3 T3前脂肪细胞因子（被认为是aP 2基因表达的阻遏物）的结合位点（4，5，7，8）。在这里，我们报告的新的互补DNA编码的3 T3前脂肪细胞因子（称为AEBP 1）的克隆，并证明AEBP 1的表达在脂肪细胞分化过程中被废除。此外，我们表明，与AEBP 1相关的羧肽酶的活性是重要的AEBP 1的转录抑制功能。因此，AEBP 1可能代表一种新型的转录因子，通过切割参与转录的因子来调节转录。
ADIPOCYTE differentiation involves the transcriptional activation of several genes in triglyceride metabolism, including the adipose P2 (aP2 or 422) gene that encodes the adipocyte lipid-binding protein ALBP(1-3). Within the mouse aP2 promoter region, the AE1 sequence functions as either a positive or a negative element in the regulation of aP2 gene expression(4-8). The AE-1 sequence is the binding site for the positive murine (3T3) adipocyte factor C/EBP-alpha(5,6), several human preadipocyte factors(7), and a 3T3 preadipocyte factor(s) that has been implicated as a repressor of aP2 gene expression(4,5,7,8). Here we report the cloning of new complementary DNAs that encode the 3T3 preadipocyte factor (termed AEBP1), and demonstrate that AEBP1 expression is abolished during adipocyte differentiation. Furthermore, we show that an activity of a carboxypeptidase associated with AEBP1 is important in the transcriptional repression function of AEBP1. Thus AEBP1 might represent a new type of transcription factor that regulates transcription by cleavage of factors involved in transcription.