Microtopographic cues for settlement of zoospores of the green fouling alga Enteromorpha
Microtopographic cues for settlement of zoospores of the green fouling alga Enteromorpha
复制标题
DOI：
10.1080/08927010290014917
复制
发表时间：
2002-09-01
期刊：
影响因子：
2.7
通讯作者：
Callow, JA
中科院分区：
文献类型：
--
作者：
Callow, ME;Jennings, AR;Callow, JA
蜈蚣是污染船只的最重要的大型藻类，它会产生大量的游动孢子，这些孢子会对许多定居线索做出反应。利用聚二甲基硅氧烷弹性体(PDMS)制成的具有定义微地形的表面，研究了表面对地形提示的响应。地形特征基于两种设计，(A)一系列5或1.5微米深的山谷，谷底和山脊在5至20微米之间变化，(B)直径5微米，高度5或1.5微米的柱子，间隔5至20微米。这些特征是分块排列的，为游动的孢子提供了在哪里定居的“选择”。游动孢子优先聚集在山谷和柱子上。随着山谷宽度的减小，定居的孢子数量大大增加。孢子主要分布在谷底与侧壁之间的夹角内。具有较低轮廓特征的曲面上的数值较小。与孢子体尺寸相似的硅珠被用来确定沉降孢子与地形特征之间的空间关系是否是主动沉降行为的结果。结果与孢子附着在不同表面特征上的能量需求有关。
Enteromorpha, the most important macroalga that fouls ships, produces very large numbers of swimming spores that respond to a number of settlement cues. Responses to topographic cues have been investigated using surfaces with defined microtopographies fabricated from polydimethyl siloxane elastomer (PDMS). The topographic features were based on two designs, (a) a series of 5 or 1.5 mum deep valleys with valley floors and ridges varying between 5 and 20 mum, and (b) pillars of 5 mum diameter and 5 or 1.5 mum height, spaced 5-20 mum apart. The features were arranged in blocks to provide the swimming spores with a 'choice' of where to settle. Swimming spores settled preferentially in the valleys and against the pillars. The number of spores that settled increased very substantially as the width of the valley decreased. The majority of spores settled in the angle between the valley floor and sidewall. Lower numbers settled on the surfaces with lower profile features. Silica beads of similar dimension to the spore body were used to determine whether the spatial relationships between settled spores and the topographic features were a consequence of active settlement behaviours. The results are discussed in relation to the energy needs for spores to adhere to various surface features.