Intracompartmental and Intercompartmental Transcriptional Networks Coordinate the Expression of Genes for Organellar Functions
Intracompartmental and Intercompartmental Transcriptional Networks Coordinate the Expression of Genes for Organellar Functions
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DOI：
10.1104/pp.111.177691
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发表时间：
2011-09-01
期刊：
影响因子：
7.4
通讯作者：
Kleine, Tatjana
中科院分区：
文献类型：
--
作者：
Leister, Dario;Wang, Xi;Kleine, Tatjana
线粒体和叶绿体蛋白质的基因分布在细胞核和细胞器基因组之间。因此，细胞器的生物发生和代谢，需要适当的协调基因表达在不同的隔间，以确保有效的合成混合遗传来源的必要的多蛋白复合物。细胞器到细胞核的信号传导在转录水平上影响核基因表达，而细胞器基因表达（OGE）被认为主要是转录后调节。在这里，我们表明，室内和室间转录网络协调细胞器功能的基因表达。近1,300 ATH 1微阵列为基础的转录模式的细胞核和细胞器基因的线粒体和叶绿体蛋白在模式植物拟南芥（拟南芥）进行了分析。参与细胞器能量产生（OEP）或OGE的基因在每个细胞器和细胞核中的活性是高度协调的。连接OEP和OGE基因组的室内网络用于同步细胞核和细胞器编码蛋白的表达。在更高的调控水平上，细胞器和核OEP/OGE基因的共表达通常调节叶绿体功能，但只有当叶绿体功能受到干扰时才会影响线粒体。在引起能量短缺的条件下，光合作用基因的室间协同调节甚至可以覆盖室内网络。我们的结论是，OEP和OGE基因的动态室内和室间转录网络调节细胞器的活性，以响应细胞的能量状态和环境压力，我们确定了候选人的顺式元件参与核基因的转录协同调节。关于叶绿体基因的转录调控，确定了新的σ因子的暂定靶基因。
Genes for mitochondrial and chloroplast proteins are distributed between the nuclear and organellar genomes. Organelle biogenesis and metabolism, therefore, require appropriate coordination of gene expression in the different compartments to ensure efficient synthesis of essential multiprotein complexes of mixed genetic origin. Whereas organelle-to-nucleus signaling influences nuclear gene expression at the transcriptional level, organellar gene expression (OGE) is thought to be primarily regulated posttranscriptionally. Here, we show that intracompartmental and intercompartmental transcriptional networks coordinate the expression of genes for organellar functions. Nearly 1,300 ATH1 microarray-based transcriptional profiles of nuclear and organellar genes for mitochondrial and chloroplast proteins in the model plant Arabidopsis (Arabidopsis thaliana) were analyzed. The activity of genes involved in organellar energy production (OEP) or OGE in each of the organelles and in the nucleus is highly coordinated. Intracompartmental networks that link the OEP and OGE gene sets serve to synchronize the expression of nucleus-and organelle-encoded proteins. At a higher regulatory level, coexpression of organellar and nuclear OEP/OGE genes typically modulates chloroplast functions but affects mitochondria only when chloroplast functions are perturbed. Under conditions that induce energy shortage, the intercompartmental coregulation of photosynthesis genes can even override intracompartmental networks. We conclude that dynamic intracompartmental and intercompartmental transcriptional networks for OEP and OGE genes adjust the activity of organelles in response to the cellular energy state and environmental stresses, and we identify candidate cis-elements involved in the transcriptional coregulation of nuclear genes. Regarding the transcriptional regulation of chloroplast genes, novel tentative target genes of sigma factors are identified.