CLONING OF A CDNA THAT ENCODES AN INVERTEBRATE GLUTAMATE RECEPTOR SUBUNIT
CLONING OF A CDNA THAT ENCODES AN INVERTEBRATE GLUTAMATE RECEPTOR SUBUNIT
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DOI：
10.1016/0014-5793(91)80846-u
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发表时间：
1991-11-04
期刊：
影响因子：
3.5
通讯作者：
DARLISON, MG
中科院分区：
文献类型：
--
作者：
HUTTON, ML;HARVEY, RJ;DARLISON, MG
利用一小段外显子序列和两个变异体的聚合酶链式反应，从池塘螺体中分离到一个编码谷氨酸受体多肽的全长cDNA。在对无脊椎动物和脊椎动物谷氨酸受体序列的首次比较中，成熟的软体动物多肽由898个氨基酸组成，预测M(R)为100 913，与大鼠亲离子谷氨酸受体亚单位GluR1至GluR6的氨基酸同源性在37%至46%之间。
A full-length cDNA which encodes a putative glutamate receptor polypeptide was isolated from the pond snail Lymnaea stagnalis, using a short stretch of exonic sequence and two variants of the polymerase chain reaction. In this first comparison of invertebrate and vertebrate glutamate receptor sequences, the mature molluscan polypeptide, which comprises 898 amino acids and has a predicted M(r) of 100 913, displays between 37% and 46% amino-acid identity to the rat ionotropic glutamate receptor subunits, GluR1 to GluR6.