Remodeling of motor terminals during metamorphosis of the moth Manduca sexta: expression patterns of two distinct isoforms of Manduca fasciclin II.
Remodeling of motor terminals during metamorphosis of the moth Manduca sexta: expression patterns of two distinct isoforms of Manduca fasciclin II.
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Manduca sexta 蛾变态过程中运动末梢的重塑：Manduca fasciclin II 两种不同亚型的表达模式。
DOI：
10.1002/cne.1165
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Kent,KS
中科院分区：
文献类型：
--
作者：
Knittel,LM;Copenhaver,PF;Kent,KS
在天蛾的变态过程中，胸足的神经肌肉系统发生了巨大的重组。幼虫的腿部肌肉在幼虫生命结束时退化，新的成虫腿部肌肉在随后的蛹阶段发育。幼虫腿部运动神经元持续存在，但中枢和外周过程经历了实质性重塑。作为我们正在进行的运动末梢重塑机制研究的一部分，我们使用针对同种粘附分子成束蛋白 II (MFas II) 的曼杜卡特异性亚型产生的抗血清来标记变态过程中的运动末梢。针对糖基磷脂酰肌醇 (GPI) 连接的 MFas II 异构体 (GPI-MFas II) 生成的抗血清标记了所有阶段的运动神经，并且似乎与包裹周围神经的神经胶质细胞有关。此外，抗 GPI-MFas II 抗血清标记的区域与幼虫和成虫阶段的突触纽扣相关。相比之下，针对 MFas II 跨膜亚型（TM-MFas II）产生的抗血清仅在重塑过程中以不连续的时间间隔标记特定的神经元过程。已确定的腿部运动神经元（例如股降肌运动神经元）仅在运动末端回缩阶段和运动末端再生长的初始阶段在其外周过程中表达可检测水平的 TM-MFas II。然而，假定的调节神经元（例如未配对的中位神经元）在幼虫阶段以及重塑过程中的过程中表达 TM-MFas II。使用异构体特异性抗 MFas II 抗血清提供了一种观察变态过程中运动末梢重塑的新方法，并有助于区分运动神经和神经肌肉接头的不同组成部分。 J.Comp。内罗尔。 434:69–85, 2001。© 2001 Wiley-Liss, Inc.
During metamorphosis of the mothManduca sexta, the neuromuscular system of the thoracic legs is reorganized dramatically. Larval leg muscles degenerate at the end of larval life, and new adult leg muscles develop during the ensuing pupal stage. Larval leg motoneurons persist, but undergo substantial remodeling of central and peripheral processes. As part of our on‐going investigation of mechanisms underlying the remodeling of motor terminals, we have used antisera generated againstManduca‐specific isoforms of the homophilic adhesion molecule fasciclin II (MFas II) to label motor terminals during metamorphosis. Antisera generated against the glycosyl‐phosphatidylinositol (GPI) ‐linked isoform of MFas II (GPI‐MFas II) labeled the motor nerves at all stages and seemed to be associated with glial cells ensheathing the peripheral nerves. In addition, the anti–GPI‐MFas II antisera labeled regions associated with synaptic boutons at both larval and adult stages. In contrast, antisera generated against a transmembrane isoform of MFas II (TM‐MFas II) only labeled specific neuronal processes at discrete intervals during remodeling. Identified leg motoneurons (such as the femoral depressor motoneuron) expressed detectable levels of TM‐MFas II in their peripheral processes only during phases of motor‐terminal retraction and initial stages of motor‐terminal re‐growth. Putative modulatory neurons (such as the unpaired median neurons), however, expressed TM‐MFas II in their processes during larval stages as well as during remodeling. Use of the isoform‐specific anti‐MFas II antisera provided a novel method for visualizing remodeling of motor terminals during metamorphosis and helped distinguish different components of the motor nerves and neuromuscular junction. J. Comp. Neurol. 434:69–85, 2001. © 2001 Wiley‐Liss, Inc.