Characterization of a member of the AN subfamily, IAN, from Ipomoea nil
Characterization of a member of the AN subfamily, IAN, from Ipomoea nil
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DOI：
10.1093/pcp/pci020
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发表时间：
2005-01-01
影响因子：
4.9
通讯作者：
Tsukaya, H
中科院分区：
文献类型：
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作者：
Cho, KH;Shindo, T;Tsukaya, H
ANGUSTIFOLIA（AN）是第一个来自植物的C-末端结合蛋白（CtBP）基因，它控制拟南芥（Arabidopsis thaliana（L.）嘿我们鉴定了一个来自Ipomoea nil（L.）罗斯（日本牵牛花），并指定它Ipomoea nil的AN（IAN）。IAN是基因组中的单拷贝基因，并且在Lnil的各种器官中普遍表达。IAN不仅包含CtBP家族中高度保守的D2-HDH基序，而且还包含AN亚家族特有的LXCXE、NLS和PEST基序。IAN cDNA的表达由花椰菜花叶病毒35 S启动子驱动，恢复了拟南芥angustifolia-1（an-1）突变体在叶宽方向上的叶扩展缺陷，表明IAN保留了与AN的共同功能。与此相反，IAN-1突变体的叶表面上的毛状体分支模式中的缺陷的互补作用不如观察到的叶形状有效。这些结果表明，AN调节叶宽和毛状体分枝的机制是可分离的。
ANGUSTIFOLIA (AN) is the first C-terminal binding protein (CtBP) gene from plants and controls leaf width and pattern of trichome branching in Arabidopsis thaliana (L.) Heynh. We characterized an ortholog of AN from Ipomoea nil (L.) Roth (Japanese morning glory) and designated it Ipomoea nil's AN (IAN). IAN is a single-copy gene in the genome and is expressed ubiquitously in various organs of L nil. IAN contains not only a D2-HDH motif, which is highly conserved within the CtBP family, but also LXCXE, NLS and PEST motifs, which are specific to the AN subfamily. The expression of IAN eDNA driven by the cauliflower mosaic virus 35S promoter restored a defect in leaf expansion in the leaf width direction in the angustifolia-1 (an-1) mutant of Arabidopsis, suggesting that IAN retains a common function with AN. In contrast, the complementation by IAN of a defect in the trichome branching pattern on the leaf surface of the an-1 mutant was less effective than that observed for leaf shape. These results suggest that the mechanisms by which AN regulates leaf width and trichome branching are separable.