Immulina as an Immunostimulatory Supplement: Formulation and Pharmacological Studies.
Immulina as an Immunostimulatory Supplement: Formulation and Pharmacological Studies.
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Immulina 作为免疫刺激补充剂：配方和药理学研究。
DOI：
10.1055/a-2156-4653
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发表时间：
2023
期刊：
影响因子：
2.7
通讯作者：
Tan,Chalet
中科院分区：
文献类型：
--
作者：
Li,Yusheng;Ji,Nan;Wang,Minia;Pugh,NirmalD;Khan,IkhlasA;Tan,Chalet
Immulina是一种市售的富含细菌脂蛋白的钝顶节旋藻提取物，可作为有效的Toll样受体2激动剂。但 Immulina免疫刺激作用在体内尚不清楚。在这里，为了设计适合于体内口服灌胃给药的Immulina制剂，Immulina纳米混悬液 制备并冷冻干燥，得到冻干的纳米Immulina，其具有约300 nm的平均粒径，并完全保留了作为Toll样受体2激动剂的生物活性。 与普通Immulina粉末相比，冻干的纳米Immulina显著加速了在水介质中的溶出。Immulina纳米悬浮液被发现刺激产生 小鼠骨髓来源的树突状细胞和巨噬细胞中的促炎细胞因子。在健康小鼠中通过纵向监测免疫应答来研究对Immulina的免疫应答。 循环中性粒细胞的吞噬活性作为替代标志物。在每日口服摄入Immulina纳米混悬液（10 mg/小鼠/天）后，循环巨噬细胞的吞噬活性增加。 中性粒细胞显著升高，提示Immulina增强先天免疫的重要机制。
Immulina is a commercially available extract ofArthrospira platensisenriched with bacterial lipoproteins that acts as a potent Toll-like receptor 2 agonist. However, the immunostimulatory effect of Immulina is not well understoodin vivo. Here, to devise an Immulina formulation suitable forin vivooral gavage dosing, Immulina nanosuspension was prepared and freeze-dried to yield lyophilized nano-Immulina, which had an average particle size of around 300 nm and fully retained the bioactivity as a Toll-like receptor 2 agonist. Compared to the regular Immulina powder, lyophilized nano-Immulina notably accelerated the dissolution in aqueous media. Immulina nanosuspension was found to stimulate the production of proinflammatory cytokines in murine bone marrow-derived dendritic cells and macrophages. The immune response to Immulina was investigated in healthy mice by longitudinally monitoring the phagocytic activity of circulating neutrophils as a surrogate marker. Following daily oral ingestion of Immulina nanosuspension (10 mg/mouse/day), the phagocytic activity of circulating neutrophils was significantly elevated, suggesting an important mechanism for Immulina to enhance innate immunity.