Oral [13C]bicarbonate measurement of CO2 stores and dynamics in children and adults.
Oral [13C]bicarbonate measurement of CO2 stores and dynamics in children and adults.
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儿童和成人口服 [13C]碳酸氢盐测量 CO2 储存和动态。
DOI：
10.1152/jappl.1990.69.5.1754
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Epstein,S
中科院分区：
文献类型：
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作者：
Armon,Y;Cooper,DM;Springer,C;Barstow,TJ;Rahimizadeh,H;Landaw,E;Epstein,S
在运动过程中，儿童每公斤体重储存的额外二氧化碳比成人少，这表明儿童储存代谢产生的二氧化碳的能力较小。为了验证这一点，10名儿童（8-12岁）和12名成人（25-40岁）在休息时口服示踪剂量的[13 C]碳酸氢盐。分析呼气中13 CO2的洗脱，以估计示踪剂的回收率、平均停留时间（MRT）和CO2储存量。还使用气体交换技术通过呼吸测量CO2产生（VCO 2）。儿童[73 +/- 13%（SD）]和成人（71 +/- 9%）之间的恢复率无显著差异。儿童MRT（42 +/- 7 min）短于成人（66 +/- 15 min，P <0.001）。儿童每千克的VCO 2（5.4 +/- 0.9 ml.min-1.kg-1）高于成人（3.1 +/- 0.5，P <0.0001）。CO2生成的示踪剂估计值与VCO 2相关（r = 0.86，P小于0.0001），当校正平均回收率时，准确预测VCO 2在3 +/-14%范围内。儿童（222 +/- 52 ml CO2/kg）和成人（203 +/- 42 ml CO2/kg）之间的静息CO2储存估计值没有差异。我们得出结论，口服[13 C]碳酸氢盐可用于评估CO2运输动力学。这些数据不支持儿童休息条件下CO2储存量较低的假设。
During exercise, less additional CO2 is stored per kilogram body weight in children than in adults, suggesting that children have a smaller capacity to store metabolically produced CO2. To examine this, tracer doses of [13C]bicarbonate were administered orally to 10 children (8-12 yr) and 12 adults (25-40 yr) at rest. Washout of 13CO2 in breath was analyzed to estimate recovery of tracer, mean residence time (MRT), and size of CO2 stores. CO2 production (VCO2) was also measured breath by breath using gas exchange techniques. Recovery did not differ significantly between children [73 +/- 13% (SD)] and adults (71 +/- 9%). MRT was shorter in children (42 +/- 7 min) compared with adults (66 +/- 15 min, P less than 0.001). VCO2 per kilogram was higher in the children (5.4 +/- 0.9 ml.min-1.kg-1) compared with adults (3.1 +/- 0.5, P less than 0.0001). Tracer estimate of CO2 production was correlated to VCO2 (r = 0.86, P less than 0.0001) and when corrected for mean recovery accurately predicted the VCO2 to within 3 +/- 14%. There was no difference in the estimate of resting CO2 stores between children (222 +/- 52 ml CO2/kg) and adults (203 +/- 42 ml CO2/kg). We conclude that orally administered [13C]bicarbonate can be used to assess CO2 transport dynamics. The data do not support the hypothesis of lower CO2 stores under resting conditions in children.