PROLIFERATION OF TOXOPLASMA-GONDII IN HUMAN NEUTROPHILS INVITRO
PROLIFERATION OF TOXOPLASMA-GONDII IN HUMAN NEUTROPHILS INVITRO
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DOI：
10.1017/s0031182000059242
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发表时间：
1991-08-01
期刊：
影响因子：
2.4
通讯作者：
KONISHI, E
中科院分区：
文献类型：
--
作者：
NAKAO, M;KONISHI, E
在体外用人中性粒细胞感染刚地弓形虫速殖子。在有特异性抗体存在和无特异性抗体存在的情况下，孵育1小时后的感染率分别为38.6 - 52.0%和16.0 - 25.8%。显然，在有抗体存在的情况下，中性粒细胞在感染18小时后杀死速殖子，而在无抗体存在时，25.0 - 35.0%的受感染中性粒细胞中的速殖子会增殖。在无抗体和补体存在的情况下，感染18小时后的速殖子总数大约是感染1小时后的4倍。补体也能增强中性粒细胞对速殖子的吞噬和杀伤作用，但其效果不如抗体。在感染后1小时内，超氧阴离子大量产生，这取决于抗体或补体的存在。在刚地弓形虫感染的初始阶段，在产生抗体之前，中性粒细胞似乎负责速殖子在宿主体内的传播。
Human neutrophils were infected with tachyzoites of Toxoplasma gondii in vitro. Infection rates after 1 h of incubation were 38.6-52.0% and 16.0-25.8% in the presence or absence of specific antibody respectively. Apparently, neutrophils killed tachyzoites 18 h after infection in the presence of antibody, whereas tachyzoites proliferated in 25.0-35.0% of infected neutrophils in the absence of antibody. Total tachyzoite counts 18 h after infection were approximately 4 times as high as those 1 h after infection in the absence of antibody and complement. Complement also enhanced phagocytosis and killing of tachyzoites by neutrophils, but the effects were less than that of antibody. Superoxide anion was produced intensively within 1 h after infection, depending on the presence of antibody or complement. Neutrophils seem to be responsible for dissemination of tachyzoites in the host during the initial phase of Toxoplasma infection until antibody is produced.