Combination of hematin and PEDOT via 1-pyrenebutanoic acid: a new platform for direct electrochemistry of hematin and biosensing applications
Combination of hematin and PEDOT via 1-pyrenebutanoic acid: a new platform for direct electrochemistry of hematin and biosensing applications
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DOI：
10.1039/c4ra05886k
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发表时间：
2014-09
期刊：
影响因子：
3.9
通讯作者：
Xuehua Yu;J. Kong;Xiaojun Han;Xueji Zhang
中科院分区：
文献类型：
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作者：
Xuehua Yu;J. Kong;Xiaojun Han;Xueji Zhang
在这项工作中，我们制备了一种基于聚（3,4-乙烯二氧噻吩）（PEDOT）和1-芘丁酸（PBA）的新型平台。PEDOT是一种杂原子掺杂的导电材料，可以通过π -π堆叠与PBA连接。通过在玻碳电极（GCE）上制备薄膜，验证了薄膜的可行性；然后，将血素通过羧酸-锆-羧酸配位键与PBA结合，制备GCE/ pedot - PBA -血素生物传感器。采用电化学阻抗谱（EIS）、循环伏安法（CV）和电流-时间曲线法（I-T）对生物传感器的电化学性能进行了测试。从CV中，观察到一对定义明确的准可逆氧化还原峰，对应于血红素Fe(III)/Fe（II）氧化还原偶对，并且计算出血红素在GCE上的表面覆盖率（Γ*）为1.2 × 10−9 mol cm−2，几乎是血红素单层覆盖率的20倍。这个值表明，PEDOT和PBA复合材料在GCE表面有更好的血红素负载。此外，GCE/ pedot - pba -血红素生物传感器对H2O2表现出较强的电催化活性，对H2O2还原在0.005 ~ 1.322 mmol L−1范围内呈线性响应，检测限为0.03 μmol L−1，灵敏度为2.83 μA mM−1 cm−2。此外，该传感器已应用于实际样品中H2O2的测定，并且响应在理想范围内，这表明GCE/ pedot - pba -血素生物传感器具有广阔的应用前景。
In this work, we prepare a novel platform based on poly(3,4-ethylenedioxythiophene) (PEDOT) and 1-pyrenebutanoic acid (PBA). PEDOT is a conductive material of heteroatom doping, which can connect with PBA through π–π stacking. The feasibility of the film is verified via fabricating it on a glassy carbon electrode (GCE); then, hematin is linked with PBA via carboxylate–zirconium–carboxylate coordination bond to prepare a GCE/PEDOT–PBA–hematin biosensor. The electrochemical performance of the biosensor has been tested by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and current-time curve method (I–T). From CV, a pair of well-defined and quasi-reversible redox peaks, corresponding to the hematin Fe(III)/Fe(II) redox couple, is observed, and the surface coverage (Γ*) of hematin on GCE has been calculated to be 1.2 × 10−9 mol cm−2, which is almost 20 times larger than the monolayer coverage of hemin. This value shows that the PEDOT and PBA composite results in a better loading of hematin on the surface of the GCE. In addition, the GCE/PEDOT–PBA–hematin biosensor exhibits strong electro-catalysis activity for H2O2 and displays a linear response for the reduction of H2O2 in the range of 0.005 to 1.322 mmol L−1 with a detection limit of 0.03 μmol L−1 and a high sensitivity of 2.83 μA mM−1 cm−2. In addition, the sensor has been applied to the determination of H2O2 in real samples, and the response is in the ideal range, which implies that the GCE/PEDOT–PBA–hematin biosensor has promising future applications.