Precocious metamorphosis in transgenic silkworms overexpressing juvenile hormone esterase
Precocious metamorphosis in transgenic silkworms overexpressing juvenile hormone esterase
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DOI：
10.1073/pnas.0500954102
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发表时间：
2005-08-16
影响因子：
11.1
通讯作者：
Shiotsuki, T
中科院分区：
文献类型：
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作者：
Tan, A;Tanaka, H;Shiotsuki, T
昆虫的生长发育受到昆虫血淋巴中保幼激素(JHS)和环状类固醇(或它们的代谢物)水平的复杂调控。JH专一性酯酶(JH-E)对JH甲酯的降解是JH降解的关键途径。在这里，我们使用二元GAL4/UAS系统产生了过表达JHE的转基因家蚕品系。从胚胎期过表达JHE导致幼虫-幼虫在第三体育场后变态，比在野生型昆虫中观察到的时间早两个体育场。这种早熟变态表明，在鳞翅目(飞蛾和蝴蝶)第二次蜕皮之前，JHS对于胚胎或幼虫的正常发育并不是至关重要的。我们的转基因方法使我们能够剖析胚胎发育过程中发生的关键生理事件的功能。到目前为止，这些类型的研究只能通过使用物理技术，如异体切除或JH类似物的应用，在较晚的幼虫阶段进行。我们相信，我们的系统将允许在昆虫生理学方面进行进一步的开创性研究。
Insect growth and development are intricately regulated by the titers of juvenile hormones (JHs) and ecclysteroids (and/or their metabolites) in the insect hemolymph. Hydrolysis of the methyl ester of JH by a JH-specific esterase (JH E) is a key pathway for the degradation of JH. Here, we generate transgenic silkworm strains that overexpress JHE by using the binary GAL4/UAS system. Overexpression of JHE from the embryonic stage resulted in larval-pupal metamorphosis after the third stadium, two stadia earlier than that observed in wild-type insects. This precocious metamorphosis suggests that JHs are not critical for normal development of embryo or larva before the second molt in Lepidoptera (moths and butterflies). Our transgenic approach allowed us to dissect the function of key physiological events that occur from embryogenesis. Until now, these types of studies were possible only in later larval stadia by using physical techniques such as allatectomy or the application of JH analogues. We believe that our system will allow further pioneering studies in insect physiology.