Increased levels of sperm protein 17 mRNA and circulating antibodies in periampullary carcinoma patients
Increased levels of sperm protein 17 mRNA and circulating antibodies in periampullary carcinoma patients
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DOI：
10.1007/s10147-014-0762-7
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发表时间：
2015-08-01
影响因子：
3.3
通讯作者：
Sharma, Rinu
中科院分区：
文献类型：
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作者：
Singh, Shivam;Saraya, Anoop;Sharma, Rinu
背景：目前检测壶腹周围癌的诊断方式是次优的，目前使用的已证实的标志物缺乏特异性和敏感性。方法采用实时荧光定量PCR技术，对壶腹周围癌患者和正常人的组织和血清中精子蛋白17的表达情况进行检测，以评估其诊断价值。此外，采用ELISA法检测壶腹周围癌患者和正常人血清中抗精子蛋白17抗体的循环水平。结果14/15例（93%）的盆腹周围癌组织中精子蛋白17表达异常（P = 0.006， Mann-Whitney U检验）。远端匹配的非恶性组织均未显示精子蛋白17mrna表达增加。曲线下面积为0.791，灵敏度为87%，特异度为73%。壶腹周围癌患者血清中精子蛋白17 mRNA水平升高（P = 0.020，学生t检验）。27/30（90%）壶腹周围癌患者血液中抗精子蛋白17抗体水平显著升高（P < 0.001, Student’st检验）。曲线下面积为0.954%，灵敏度为86.7%，特异度为96.3%。只有两名正常受试者（7%）显示抗精子蛋白17抗体水平升高。结论本研究首次提示，高水平的精子蛋白17 mRNA和高水平的循环抗精子蛋白17抗体可用于区分壶腹周围癌患者和健康个体，突出了精子蛋白17的诊断潜力。
Background Present-day diagnostic modalities for detecting periampullary carcinoma are suboptimal, and currently used proven markers lack specificity and sensitivity.Methods In order to assess the diagnostic potential of sperm protein 17, a cancer testis antigen, quantitative real-time PCR was performed to evaluate the expression of sperm protein 17 in tissue and sera specimens collected from periampullary carcinoma patients and normal subjects. Additionally, circulating levels of anti-sperm protein 17 antibodies were determined in sera of periampullary carcinoma patients and normal subjects using ELISA.Results Aberrant expression of sperm protein 17 was found in 14/15 (93 %) periampullary cancer tissues when compared with distant matched nonmalignant tissues (P = 0.006, Mann-Whitney U test). None of the distant matched nonmalignant tissues showed increased expression of sperm protein 17 mRNA. Area under the curve, sensitivity, and specificity were 0.791, 87, and 73 %, respectively. Increased levels of sperm protein 17 mRNA were demonstrated in sera of periampullary carcinoma patients (P = 0.020, Student's t test). Circulating levels of anti-sperm protein 17 antibody were found to be significantly elevated in 27/30 (90 %) periampullary carcinoma patients (P < 0.001, Student's t test). Area under the curve, sensitivity, and specificity were 0.954, 86.7, and 96.3 %, respectively. Only two of the normal subjects (7 %) showed elevated levels of anti-sperm protein 17 antibody.Conclusion For the first time, our findings suggest that high levels of sperm protein 17 mRNA as well as increased circulating anti-sperm protein 17 antibodies can be used to distinguish periampullary cancer patients from healthy individuals, highlighting the diagnostic potential of sperm protein 17.