Effects of salinity and temperature on the timing of germinal vesicle breakdown and polar body release in diploid and triploid Hong Kong oysters, Crassostrea hongkongensis , in relation to tetraploid induction
Effects of salinity and temperature on the timing of germinal vesicle breakdown and polar body release in diploid and triploid Hong Kong oysters, Crassostrea hongkongensis , in relation to tetraploid induction
复制标题
DOI：
10.1111/are.13833
复制
发表时间：
2018-09
影响因子：
2
通讯作者：
Yanping Qin;Shu Xiao;Haitao Ma;Riguan Mo;Zihua Zhou;Xiangwei Wu;Yuehuan Zhang;Ziniu Yu
中科院分区：
文献类型：
--
作者：
Yanping Qin;Shu Xiao;Haitao Ma;Riguan Mo;Zihua Zhou;Xiangwei Wu;Yuehuan Zhang;Ziniu Yu
四倍体对全三倍体香港长牡蛎的商业化生产至关重要。四倍体idc的成功生产。香港需要精确控制施肥和施用处理。受精前剥卵胚泡破裂率（GVBD）影响四倍体诱导效率。根据我们的研究，在二倍体和三倍体中，如果在受精前GVBD比例达到100%，极体1 （PB1）释放到50%的时间显著缩短（p< 0.05）。我们认为剥离的卵母细胞有100% GVBD的时间是开始受精的最佳时间点。50%的PB1释放是应用诱导四倍体处理的重要时间点。由于降低盐度（25 ~ 15ppt）和升高温度（25 ~ 31℃）都能加快GVBD和卵PB1的释放速度，31℃和15℃是进行四倍体诱导的最合适的温度和盐度水平。hongkongensis。在这些条件下，我们发现不同三倍体的卵在100% GVBD和50% PB1释放时间上的差异明显大于不同二倍体（p< 0.05），这给三倍体卵诱导四倍体的受精和处理时间的控制带来了困难。为了减少变异，我们推荐四倍体诱导的处理。香港应该使用三倍体雌性个体的卵。
Tetraploids are crucial important for commercial production of all‐triploidCrassostrea hongkongensis. Successful production of tetraploidC. hongkongensisrequires precise control of fertilization and applying treatment. The germinal vesicle breakdown (GVBD) ratio of stripped eggs before fertilization influences the efficiency of tetraploid inductions. According to our research, in both diploids and triploids, the time to 50% polar body 1 (PB1) release was significantly reduced if a GVBD ratio of 100% was reached before fertilization (p< 0.05). We consider the time at which there is 100% GVBD of stripped oocytes to be the optimum time point to start fertilization. 50% PB1 release is an important time point to apply the treatment for inducing tetraploids. Since lowering salinity (from 25 to 15 ppt) and increasing temperature (from 25 to 31°C) both accelerate the GVBD and the PB1 release rate of eggs, 31°C and 15 ppt are the most appropriate temperature and salinity level in which to perform tetraploid induction ofC. hongkongensis. In these conditions, we found that there was significantly more variation in timing of 100% GVBD and 50% PB1 release among eggs from different triploids compared to different diploids (p< 0.05), creating difficulties in controlling fertilization and duration of treatment in tetraploid induction using triploid eggs. For reducing the variation, we recommend that treatments for tetraploid induction ofC. hongkongensisshould use eggs from individual triploid females.