Genomic transcriptional profiling of the developmental cycle of Chlamydia trachomatis
Genomic transcriptional profiling of the developmental cycle of Chlamydia trachomatis
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DOI：
10.1073/pnas.1331135100
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发表时间：
2003-07-08
影响因子：
11.1
通讯作者：
Caldwell, HD
中科院分区：
文献类型：
--
作者：
Belland, RJ;Zhong, GM;Caldwell, HD
沙眼衣原体是最常见的细菌性病原体之一，是人类衰弱性传播疾病和眼部疾病的病原。该生物是一种专性细胞内原核生物，具有高度特化的双相发育周期。我们对衣原体发育周期进行了基因组转录分析。这种方法已经确定了一小部分基因，这些基因控制着周期的初级（即早期基因）和次级（即晚期基因）分化阶段。直接早期基因产物启动细菌代谢，并可能修饰细菌吞噬体以逃避与溶酶体的融合。一个直接早期基因（CT147）是人类早期内体抗原-1的同源物，定位于衣原体吞噬体；这表明CT147在非融合途径中建立寄生物液泡的功能作用。晚期基因产物终止细菌细胞分裂，并构成结构成分和重塑活动，参与形成高度二硫交联的外膜复合物，该复合物在附着和入侵新宿主细胞中起作用。在分化早期和晚期表达的许多基因是衣原体特异性的，并且在真核生物谱系中具有进化起源。
Chlamydia trachomatis is one of the most common bacterial pathogens and is the etiological agent of debilitating sexually transmitted and ocular diseases in humans. The organism is an obligate intracellular prokaryote characterized by a highly specialized biphasic developmental cycle. We have performed genomic transcriptional analysis of the chlamydial developmental cycle. This approach has led to the identification of a small subset of genes that control the primary (immediate-early genes) and secondary (late genes) differentiation stages of the cycle. Immediate-early gene products initiate bacterial metabolism and potentially modify the bacterial phagosome to escape fusion with lysosomes. One immediate early gene (CT147) is a homolog of the human early endosomal antigen-1 that is localized to the chlamydial phagosome; suggesting a functional role for CT147 in establishing the parasitophorous vacuole in a nonfusogenic pathway. Late gene products terminate bacterial cell division and constitute structural components and remodeling activities involved in the formation of the highly disulfide cross-linked outer-membrane complex that functions in attachment and invasion of new host cells. Many of the genes expressed during the immediate-early and late differentiation stages are Chlamydia-specific and have evolutionary origins in eukaryotic lineages.