Novel approaches for studying pulmonary toxicity in vitro
Novel approaches for studying pulmonary toxicity in vitro
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DOI：
10.1016/s0378-4274(02)00512-x
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发表时间：
2003-04-11
影响因子：
3.5
通讯作者：
Ritter, D
中科院分区：
文献类型：
--
作者：
Aufderheide, M;Knebel, JW;Ritter, D
由于将呼吸道培养细胞直接暴露于可能包括复杂气体和颗粒混合物的测试气氛的困难，对吸入污染物诱导的不利细胞效应的体外研究提出了一个特殊问题。一般来说，没有广泛接受的体外暴露系统。然而，体外方法提供了使用人细胞的独特可能性，开发并验证了使用空气/液体暴露技术原理的细胞培养和暴露装置（CULTEX 1）。呼吸道细胞生长在transwell插入物的多孔膜上。除去培养基后，可以用测试气氛在细胞的表面上处理细胞，同时通过下面的膜向它们提供营养。与其他实验方法相比，我们研究的目标是分析环境条件下测试气氛的生物效应，即不破坏大气或添加额外的CO2。所使用的系统是足够小和灵活的独立的培养室，从而提供了室内的现场研究的机会。和野外的大气层。暴露装置的有效性已经在上皮肺细胞暴露于复杂混合物（如天然柴油机废气和侧流烟雾）的剂量依赖性细胞毒性和遗传毒性效应分析中得到证实。(C)2003爱思唯尔科学爱尔兰有限公司保留所有权利。
The in vitro study of adverse cellular effects induced by inhaled pollutants poses a special problem due to the difficulties of exposing cultured cells of the respiratory tract directly to test atmospheres that can include complex gaseous and particulate mixtures. In general, there is no widely accepted in vitro exposure system. However, in vitro methods offer the unique possibility for use of human cells, developed and validated cell culture and exposure device (CULTEX1) using the principle of the air/liquid exposure technique. Cells of the respiratory tract are grown on porous membranes in transwell inserts. After removal of the medium, the cells can be treated on their superficial surfaces with the test atmosphere, and at the same time they are supplied with nutrients through the membrane below. In comparison with other experimental approaches, the goal of our studies is to analyze the biological effects of test atmospheres under environmental conditions, i.e. without humidifying the atmosphere or adding additional CO2. The system used is small and flexible enough independent of a cultivation chamber and thus offers the opportunity for onsite study of indoor. and outdoor atmospheres in the field. The efficacy of the exposure device has already been demonstrated in the analysis of dose-dependent cytotoxic and genotoxic effects of exposure of epithelial lung cells to complex mixtures such as native diesel exhaust and side-stream smoke. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.