Effects of anti-CD4 antibody treatment on calcium ions influx in peanut-sensitized C3H/HeJ mice
Effects of anti-CD4 antibody treatment on calcium ions influx in peanut-sensitized C3H/HeJ mice
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DOI：
10.1016/j.fshw.2022.09.010
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发表时间：
2023-05-01
影响因子：
7
通讯作者：
Che，Huilian
中科院分区：
文献类型：
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作者：
Wang，Junjuan;Zhou，Cui;Che，Huilian
抗CD4抗体和钙离子(Ca~(2+))在花生过敏中的确切作用机制尚不清楚。用花生蛋白提取物(PPE)致敏的C3H/HeJ小鼠注射抗CD4抗体4周。PPE刺激后，脾组织特异性免疫球蛋白(IgE)、细胞因子、组胺和mMcp-1水平升高，核心蛋白核心蛋白(DCN)表达上调，脾细胞内钙内流，转录因子GATA-3和Foxp3表达增强，Th2和Treg细胞活化。值得注意的是，Ca~(2+)水平与组胺、IL-4、IL-5、IL-13水平呈正相关，与IL-10水平呈负相关。然而，抗CD4抗体可显著减轻过敏症状，激活T细胞，减少钙离子内流、细胞因子、组胺、mMcp-1以及IgHG3、CXCL12、MMP2和FABP4基因。我们的结果表明，抗CD4抗体可以减轻PPE诱导的过敏反应，这可能与其抑制Ca~(2+)内流，抑制组胺、细胞因子和IgHG3、CXCL12、MMP2和FABP4有关，从而对PPE致敏的食物过敏起到保护作用。
The precise mechanism underlying the effects of anti-CD4 antibody and calcium ions (Ca2+) in peanut allergy remains unknown. C3H/HeJ mice sensitized with peanut protein extract (PPE) were injected with anti-CD4 antibodies for 4 weeks. Stimulation with PPE increased the specific immunoglobulin (IgE), cytokine, histamine, and mMcp-1 levels, upregulated decorin (Dcn) expression, induced Ca2+inflow in the spleen, and augmented the expression of the transcription factors GATA-3 and Foxp3, which resulted in Th2 and Treg cell activation. Notably, the Ca2+levels were positively correlated with the histamine, interleukin (IL)-4, IL-5, and IL-13 levels, and negatively correlated with IL-10 levels. However, administration of anti-CD4 antibodies markedly alleviated allergic symptoms, activated T cells, and reduced Ca2+inflow, cytokine, histamine, mMcp-1, and the IgHG3, CXCL12, MMP2 and FABP4 gene. Our results indicated that anti-CD4 antibodies can ameliorate PPE-induced allergy, which is probably related to the suppression of Ca2+inflow, and inhibiting histamine, cytokine and IgHG3, CXCL12, MMP2, and FABP4, thus exerting a protective effect against PPE-sensitized food allergy.