Plant growth promotion and suppression of bacterial leaf blight in rice by Paenibacillus polymyxa Sx3
Plant growth promotion and suppression of bacterial leaf blight in rice by Paenibacillus polymyxa Sx3
复制标题
多粘类芽孢杆菌Sx3促进植物生长并抑制水稻白叶枯病
DOI：
10.1111/lam.13117
复制
发表时间：
2019-05-01
影响因子：
2.4
通讯作者：
Li, B.
中科院分区：
文献类型：
--
作者：
Abdallah, Y.;Yang, M.;Li, B.
研究了多粘类芽孢杆菌Sx 3对水稻白叶枯病的促生和抑制作用及其机理。平板试验结果表明，Sx 3对20株黄单胞菌的生长有抑制作用。水稻（Xoo）。水稻苗期试验表明，Sx 3能促进植株生长，抑制白叶枯病。此外，细菌学试验表明，Sx 3能够固定氮，溶解磷酸盐和产生吲哚乙酸，表明多种机制可能参与Sx 3的生长促进。透射电镜和扫描电镜观察表明，多粘类囊藻Sx 3培养滤液能抑制细菌生长、生物膜形成并破坏Xoo菌株GZ 0005的细胞形态。此外，MALDI-TOF MS分析显示，Sx 3可以生物合成两种类型的次级代谢产物杀镰孢菌素和多粘菌素P。总之，本研究清楚地表明，P. polymyxa Sx 3对Xoo具有较强的体外和体内拮抗活性，这可能至少部分归因于其次级代谢产物的产生。
The effects and mechanisms of Paenibacillus polymyxa Sx3 on growth promotion and the suppression of bacterial leaf blight in rice were evaluated in this study. The results from a plate assay indicated that Sx3 inhibited the growth of 20 strains of Xanthomonas oryzae pv. oryzae (Xoo). Rice seedling experiments indicated that Sx3 promoted plant growth and suppressed bacterial leaf blight. In addition, bacteriological tests showed that Sx3 was able to fix nitrogen, solubilize phosphate and produce indole acetic acid, indicating that various mechanisms may be involved in the growth promotion by Sx3. The culture filtrate of P. polymyxa Sx3 reduced bacterial growth, biofilm formation and disrupted the cell morphology of Xoo strain GZ 0005, as indicated by the transmission and scanning electron microscopic observations. In addition, MALDI‐TOF MS analysis revealed that Sx3 could biosynthesize two types of secondary metabolites fusaricidins and polymyxin P. In summary, this study clearly indicated that P. polymyxa Sx3 has strong in vitro and in vivo antagonistic activity against Xoo, which may be at least partially attributed to its production of secondary metabolites.