The effect of growth hormone and insulin upon MLC responses and the generation of cytotoxic lymphocytes.
The effect of growth hormone and insulin upon MLC responses and the generation of cytotoxic lymphocytes.
复制标题
生长激素和胰岛素对 MLC 反应和细胞毒性淋巴细胞生成的影响。
作者：
E. Snow;T. Feldbush;J. Oaks
通过使用血清替代系统，分析了生长激素 (GH) 和胰岛素对体外同种异体抗原刺激作出积极反应的 T 淋巴细胞的能力。胰岛素而非 GH 的存在能够成功产生混合淋巴细胞培养物 (MLC) 胚细胞反应。然而，在 5 天的 MLC 期间 GH 的存在允许细胞毒性 T 淋巴细胞 (CTL) 的产生。进一步证明，GH 需要在培养系统的前 2 天存在，大概是在前体 CTL 进入细胞分裂之前。从 GH 诱导鸟氨酸脱羧酶活性以及这如何与 CTL 活性的成功产生相关方面讨论了结果。
The ability of growth hormone (GH) and insulin to influence positively T lymphocytes responding to an alloantigen stimulus in vitro was analyzed through the use of a serum substitute system. The presence of insulin but not GH, enables the generation of a successful mixed lymphocyte culture (MLC) blastogenic response. However, the presence of GH during a 5-day MLC allowed for the generation of cytotoxic T lymphocytes (CTL). It was further demonstrated that GH needed to be present during the first 2 days of the culture system, presumably before the entry of the precursor CTL into cell division. The results are discussed in terms of the induction, by the GH, of ornithine decarboxylase activity and how this might relate to the successful generation of CTL activity.