Tissue engineering for full-thickness burns: A dermal substitute from bench to bedside
Tissue engineering for full-thickness burns: A dermal substitute from bench to bedside
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DOI：
10.1016/j.bbrc.2007.08.155
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发表时间：
2007-11-23
影响因子：
3.1
通讯作者：
Dosquet, Christine
中科院分区：
文献类型：
--
作者：
Kellouche, Sabrina;Martin, Christophe;Dosquet, Christine
我们的目的是获得一种可行且易于获得的真皮替代品（DS），以确定全层烧伤的覆盖范围。描述了一种由胶原-糖胺聚糖-壳聚糖真皮基质（DM）组成的包皮成纤维细胞定殖的DS（1717）。将ff定殖的DS与成人真皮成纤维细胞（DF）播种的DM进行比较。ff定殖的DS比DF定殖的DS表达更多的原纤维蛋白和对弹力蛋白。用FF和df定殖的DS获得的重建皮肤在真皮-表皮交界处同样表达层粘连蛋白-5和胶原VII。在血小板裂解液存在的情况下，FF和df定植的DS均以剂量依赖的方式产生皮肤伤口愈合介质。冻融后，ff定殖的DS在培养中恢复，并保留了产生血管内皮生长因子的能力。裸鼠移植DS后，真皮-表皮病变完全愈合，表皮化良好。(C) 2007爱思唯尔公司版权所有。
Our aim was to obtain a viable and easily available dermal substitute (DS) for the definitive coverage of full-thickness burns. A DS composed of a collagen-glycosaminoglycan-chitosan dermal matrix (DM) colonized with foreskin fibroblasts (1717) is described. FF-colonized DS were compared to the DM seeded with adult dermal fibroblasts (DF). FF-colonized DS expressed more fibrillin and tropoelastin than that with DF. Reconstructed skin obtained with both FF- and DF-colonized DS similarly expressed laminin-5 and collagen VII at the dermal-epidermal junction. Both FF- and DF-colonized DS produced cutaneous wound healing mediators in a dose-dependent manner in the presence of platelet lysate. After freeze-thawing, the FF-colonized DS were recovered in culture and retained their ability to produce vascular endothelial growth factor. Grafting of DS into nude rats achieved a complete healing of a dermal-epidermal lesion with a good epidermalization. (C) 2007 Elsevier Inc. All rights reserved.