Identification of grapevine MLO gene candidates involved in susceptibility to powdery mildew
Identification of grapevine MLO gene candidates involved in susceptibility to powdery mildew
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DOI：
10.1071/fp08173
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发表时间：
2008-01-01
影响因子：
3
通讯作者：
Dry, Ian B.
中科院分区：
文献类型：
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作者：
Feechan, Angela;Jermakow, Angelica M.;Dry, Ian B.
欧洲栽培葡萄藤，葡萄属葡萄属L.，是葡萄栽培中最重要的经济真菌病害白粉病病原体葡萄瘿螨（ErisypheNecator）的宿主。MLO蛋白介导模式植物物种拟南芥和作物植物大麦和番茄中的白粉病易感性。从葡萄藤中分离出7个VvMLO cDNA序列，随后鉴定为酿酒葡萄基因组内17个成员的VvMLO基因家族的一部分。对葡萄基因组中17个VvMLO基因的系统发育分析表明，它们编码的蛋白质属于6个不同的进化枝。在一系列葡萄组织中，以及在响应一系列生物和非生物因素时，分析了来自每个进化枝的代表性VvMLO的表达。研究的VvMLO具有独特但重叠的组织表达模式。VvMLO基因在E. necator感染鉴定了四个上调的VvMLO，它们与拟南芥AtMLO 2、AtMLO 6和AtMLO 12以及番茄SlMLO 1基因是白粉病易感性所需的。这表明由这些基因编码的蛋白质在对白粉病的易感性方面存在一定程度的功能冗余，因此代表了modi的潜在靶标。阳离子以产生抗白粉病的葡萄藤。
The European cultivated grapevine, Vitis vinifera L., is a host for the powdery mildew pathogen Erisyphe necator, which is the most economically important fungal disease of viticulture. MLO proteins mediate powdery mildew susceptibility in the model plant species Arabidopsis and the crop plants barley and tomato. Seven VvMLO cDNA sequences were isolated from grapevine and were subsequently identified as part of a 17 member VvMLO gene family within the V. vinifera genome. Phylogenetic analysis of the 17 VvMLO genes in the grape genome indicated that the proteins they encode fall into six distinct clades. The expression of representative VvMLOs from each clade were analysed in a range of grape tissues, as well as in response to a range of biotic and abiotic factors. The VvMLOs investigated have unique, but overlapping tissue expression patterns. Expression analysis of VvMLO genes following E. necator infection identified four upregulated VvMLOs which are orthologous to the Arabidopsis AtMLO2, AtMLO6 and AtMLO12 and tomato SlMLO1 genes required for powdery mildew susceptibility. This suggests a degree of functional redundancy between the proteins encoded by these genes in terms of susceptibility to powdery mildew, and, as such, represent potential targets for modi. cation to generate powdery mildew resistant grapevines.