Activity of the Arabidopsis RD29A and RD29B promoter elements in soybean under water stress
Activity of the Arabidopsis RD29A and RD29B promoter elements in soybean under water stress
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DOI：
10.1007/s00425-012-1740-9
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发表时间：
2013-01-01
期刊：
影响因子：
4.3
通讯作者：
Clemente, Tom E.
中科院分区：
文献类型：
--
作者：
Bihmidine, Saadia;Lin, Jiusheng;Clemente, Tom E.
通过与视觉标记基因 β-葡萄糖醛酸酶 (GUS) 融合，监测大豆 (Glycine max (L.) Merr.] 中拟南芥 RD29A 和 RD29B 启动子的组成型和干旱诱导活性。在温室条件下缺水 9 天期间监测大豆植物的生理反应。使用数据选择适当的时间点来监测各个启动子元件的活性。定性和定量在水分充足和干燥条件下的植物中，RD29A 和 RD29B 启动子均在根部和叶组织中显着激活，然而，在水分充足条件下的植物根和叶中也观察到低水平的组成型启动子活性，这些观察结果表明 RD29X 中存在相应的干旱响应调控元件。启动子可用于控制靶向转基因以减轻大豆中的非生物胁迫，前提是在这些启动子控制下的转基因在未施加非生物胁迫时不会因表达渗漏而引起农艺惩罚。
The constitutive and drought-induced activities of the Arabidopsis thaliana RD29A and RD29B promoters were monitored in soybean (Glycine max (L.) Merr.] via fusions with the visual marker gene beta-glucuronidase (GUS). Physiological responses of soybean plants were monitored over 9 days of water deprivation under greenhouse conditions. Data were used to select appropriate time points to monitor the activities of the respective promoter elements. Qualitative and quantitative assays for GUS expression were conducted in root and leaf tissues, from plants under well-watered and dry-down conditions. Both RD29A and RD29B promoters were significantly activated in soybean plants subjected to dry-down conditions. However, a low level of constitutive promoter activity was also observed in both root and leaves of plants under well-watered conditions. GUS expression was notably higher in roots than in leaves. These observations suggest that the respective drought-responsive regulatory elements present in the RD29X promoters may be useful in controlling targeted transgenes to mitigate abiotic stress in soybean, provided the transgene under control of these promoters does not invoke agronomic penalties with leaky expression when no abiotic stress is imposed.