Engineering Protein Hydrogels Using SpyCatcher-SpyTag Chemistry
Engineering Protein Hydrogels Using SpyCatcher-SpyTag Chemistry
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DOI：
10.1021/acs.biomac.6b00566
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发表时间：
2016-09-01
影响因子：
6.2
通讯作者：
Li, Hongbin
中科院分区：
文献类型：
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作者：
Gao, Xiaoye;Fang, Jie;Li, Hongbin
从工程蛋白质构建水凝胶已经引起了材料科学界的极大关注，因为它们在生物医学工程中有无数潜在的应用。开发将定制的蛋白质构建块交联成具有期望的机械、物理和功能性质的水凝胶的有效方法是至关重要的。通过利用最近开发的SpyCatcher-SpyTag化学，我们成功地在工程化串联模块弹性蛋白的基础上工程化蛋白质水凝胶。我们得到的蛋白质水凝胶柔软但稳定，并显示出优异的生物相容性。作为第一步，我们测试了这些水凝胶作为药物载体的用途，以及封装人肺成纤维细胞。我们的研究结果表明的SpyCatcher-SpyTag化学的鲁棒性，即使当SpyTag（或SpyCatcher）两侧折叠球状域。这些结果表明，SpyCatcher-SpyTag化学可用于从串联模块化弹性体蛋白质工程化蛋白质水凝胶，其可在组织工程、基础机械生物学研究和作为受控药物释放载体中找到应用。
Constructing hydrogels from engineered proteins has attracted significant attention within the material sciences, owing to their myriad potential applications in biomedical engineering. Developing efficient methods to cross-link tailored protein building blocks into hydrogels with desirable mechanical, physical, and functional properties is of paramount importance. By making use of the recently developed SpyCatcher-SpyTag chemistry, we successfully engineered protein hydrogels on the basis of engineered tandem modular elastomeric proteins. Our resultant protein hydrogels are soft but stable, and show excellent biocompatibility. As the first step, we tested the use of these hydrogels as a drug carrier, as well as in encapsulating human lung fibroblast cells. Our results demonstrate the robustness of the SpyCatcher-SpyTag chemistry, even when the SpyTag (or SpyCatcher) is flanked by folded globular domains. These results demonstrate that SpyCatcher-SpyTag chemistry can be used to engineer protein hydrogels from tandem modular elastomeric proteins that can find applications in tissue engineering, in fundamental mechano-biological studies, and as a controlled drug release vehicle.