Asymmetric reduction of ketones using recombinant E-coli, cells that produce a versatile carbonyl reductase with high enantioselectivity and broad substrate specificity
Asymmetric reduction of ketones using recombinant E-coli, cells that produce a versatile carbonyl reductase with high enantioselectivity and broad substrate specificity
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DOI：
10.1016/j.tet.2006.04.061
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发表时间：
2006-06-26
期刊：
影响因子：
2.1
通讯作者：
Sakai, Takashi
中科院分区：
文献类型：
--
作者：
Ema, Tadashi;Yagasaki, Hideo;Sakai, Takashi
一种对多种酮类具有高对映选择性的多功能生物催化剂SCR （Saccharomyces cerevisiae羰基还原酶）基因已被鉴定、克隆并在大肠杆菌中表达。构建了两种具有高nadph再生能力的表达系统。一种是串联系统，其中编码SCR和GDH（葡萄糖脱氢酶）的基因位于同一个质粒中；另一种是双质粒系统，其中每个SCR和GDH基因位于可以共存于一个大肠杆菌细胞中的不同质粒中。用重组大肠杆菌细胞对酮类进行不对称还原，得到合成有用的20种醇，其中11种是对映体纯醇。其中一种产品的产率高达41 g/L。(c) 2006 Elsevier Ltd.版权所有。
The gene encoding a versatile biocatalyst that shows high enantioselectivity for a variety of ketones, SCR (Saccharomyces cerevisiae carbonyl reductase), has been identified, cloned, and expressed in Escherichia coli. Two types of expression systems with high NADPH-regenerating capacities have been constructed. One is the tandem system, where the genes encoding SCR and GDH (glucose dehydrogenase) are located in the same plasmid, and the other is the two-plasmid system, where each of the SCR and GDH genes is located in separate plasmids that can coexist in one E. coli cell. Asymmetric reduction of ketones with the recombinant E. coli cells gave synthetically useful 20 alcohols, 11 of which were enantiomerically pure. The productivity of one of these products was as high as 41 g/L. (c) 2006 Elsevier Ltd. All rights reserved.