Isopentylamine is a novel defence compound induced by insect feeding in rice
Isopentylamine is a novel defence compound induced by insect feeding in rice
复制标题
DOI：
10.1111/pce.13902
复制
发表时间：
2020-10-16
影响因子：
7.3
通讯作者：
Murayama, Tetsuya
中科院分区：
文献类型：
--
作者：
Aboshi, Takako;Iitsuka, Chiaki;Murayama, Tetsuya
植物产生各种各样的防御代谢物来保护自己免受草食性昆虫的侵害。虽然多胺已被牵连在各种非生物和生物胁迫的反应，有没有研究集中在胺响应昆虫植食性。通过筛选具有生物活性的胺类化合物，我们在稳定同位素标记实验中鉴定出异戊胺是水稻叶片中一种新型的草食诱导化合物，其来源于氨基酸亮氨酸。异戊胺的积累增加，在植食性的褐飞虱（褐飞虱，BPH）和水稻饲养粘虫（Mythimna loreyi），以及在响应处理与植物激素，茉莉酸。同样，异戊胺的积累在水稻茉莉酸生物合成突变体hebiba和Osjar 1中受到损害。在生物测定中，取食50 mg/L异戊胺浸泡水稻幼苗的褐飞虱死亡率高于取食清水浸泡水稻幼苗的褐飞虱。50 mg/L溶液浸泡水稻叶片，其内源异戊胺浓度与BPHs诱导的浓度相似。这些结果表明，异戊胺是一种新型的植物防御代谢产物，可被植食性昆虫快速诱导，并对水稻中的植食性昆虫产生威慑作用。
Plants produce a broad variety of defensive metabolites to protect themselves against herbivorous insects. Although polyamines have been implicated in various responses to abiotic and biotic stress, there have been no studies focused on amines in response to insect herbivory. By screening for bioactive amines, we identified isopentylamine as a novel type of herbivory-induced compound in rice leaves, which was derived from the amino acid leucine in stable isotope labelling experiments. Accumulation of isopentylamine increased during herbivory by the brown planthopper (Nilaparvata lugens, BPH) and the rice-feeding armyworm (Mythimna loreyi), as well as in response to treatment with the plant hormone, jasmonic acid. Likewise, isopentylamine accumulation was compromised in rice jasmonate biosynthesis mutants,hebibaandOsjar1. In bio-assays, BPH insects feeding on rice seedlings submerged in 50 mg/L isopentylamine solution had a higher mortality compared with BPH feeding on seedlings submerged in water. Notably, the rice leaves submerged in 50 mg/L solution showed the endogenous concentrations of isopentylamine similar to that induced by BPHs. These results suggest that isopentylamine functions as a new type of plant defence metabolite that is rapidly induced by herbivore attack and deters insect herbivores in rice.