Stimulation of phospholipase D activity and phosphatidic acid production by norepinephrine in rat aorta.
Stimulation of phospholipase D activity and phosphatidic acid production by norepinephrine in rat aorta.
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去甲肾上腺素刺激大鼠主动脉中磷脂酶 D 的活性和磷脂酸的产生。
DOI：
10.1152/ajpcell.1993.264.3.c609
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Geisbuhler,BB
中科院分区：
文献类型：
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作者：
Jones,AW;Shukla,SD;Geisbuhler,BB
我们试图将去甲肾上腺素（NE）刺激磷脂酸（PA）的产生与大鼠主动脉的功能反应和PA产生的途径联系起来。PA变化的时间过程与42K外排和收缩的钙依赖性强直反应密切相关。根据PI分析和磷脂32P标记，NE（30微米，1分钟）增加了PA，降低了磷脂酰胆碱（PC）和磷脂酰肌醇（PI）。PA的32p / PI比值（0.8 +/- 0.2,n = 13）与PC （0.8 +/- 0.1, n = 14）相似，但显著低于PI （4.6 +/- 0.5, n = 14）。与磷酸磷脂酰肌醇和二磷酸磷脂酰肌醇相比，磷酸磷脂酰肌醇的32p / pi比值也较低（P < 0.02）。当PC选择性标记[3H]肉豆蔻酸时，NE使[3H]PA增加了2倍（P < 0.05）。这些观察结果更符合磷脂酶D （PLD）水解PC形成的PA，而不是磷脂酶c作用下产生的二酰基甘油磷酸化形成的PA。PLD是通过与乙醇的转磷脂酰化反应形成磷脂酰乙醇（PEt）来测定的（半最大刺激在0.4-0.5% vol/vol）。NE刺激PLD的时间过程与PA相似，在10 s至30 min期间显著增加（P < 0.002）。PEt一旦形成，降解缓慢，一半时间为bbb30 h。由此可见，NE刺激大鼠主动脉PLD，使PC水解形成大量PA。（摘要删节250字）
We sought to relate norepinephrine (NE) stimulation of phosphatidic acid (PA) production to functional responses of rat aorta and pathways for PA production. The time course for changes in PA was closely related to Ca-dependent tonic responses in 42K efflux and contraction. NE (30 microM for 1 min) increased PA and reduced phosphatidylcholine (PC) and phosphatidylinositol (PI) based on Pi analyses and 32P labeling of phospholipids. The 32P-to-Pi ratio in PA (0.8 +/- 0.2, n = 13) was similar to PC (0.8 +/- 0.1, n = 14) but was significantly lower (P < 0.001) than PI (4.6 +/- 0.5, n = 14). The 32P-to-Pi ratio in PA was also lower (P < 0.02) than phosphatidylinositol phosphate and phosphatidylinositol bisphosphate. NE also increased [3H]PA twofold (P < 0.05) when PC was selectively labeled with [3H]myristic acid. These observations are more consistent with PA being formed from the hydrolysis of PC by phospholipase D (PLD) than by the phosphorylation of diacylglycerol produced by the action of phospholipase C. PLD was assayed by the formation of phosphatidylethanol (PEt) via a transphosphatidylation reaction with ethanol (half-maximal stimulation at 0.4-0.5% vol/vol). The time course for PLD stimulation by NE was similar to PA, with significant increases (P < 0.002) during 10 s to 30 min exposure. Once formed, PEt was degraded slowly, with a half time > 3 h. It is concluded that NE stimulates PLD in rat aorta, which forms a significant amount of PA from the hydrolysis of PC.(ABSTRACT TRUNCATED AT 250 WORDS)