Differential macrophage infiltration in early and advanced endometriosis and adjacent peritoneum
Differential macrophage infiltration in early and advanced endometriosis and adjacent peritoneum
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DOI：
10.1016/j.fertnstert.2003.07.037
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发表时间：
2004-03-01
影响因子：
6.7
通讯作者：
Ishimaru, T
中科院分区：
文献类型：
--
作者：
Khan, KN;Masuzaki, H;Ishimaru, T
目的：探讨巨噬细胞(MO)在在位和异位内膜在月经周期中的分布。设计：采用完整组织进行临床对照研究。地点：日本长崎长崎大学医学院。患者(S)，20例盆腔子宫内膜异位症不孕症患者和20例非子宫内膜异位症患者。干预(S)：取自有或无内异症患者的腹膜病变和相应在位内膜的活检标本。邻近的腹膜活检也取自这些妇女中的一小部分。对活化的MPHI标志物CD68、促有丝分裂标志物肝细胞生长因子、内皮细胞表面标志物von Willebrand因子进行免疫定位和Q-H评分。主要观察指标(S)：观察MPHI在在位内膜、异位内膜及邻近腹膜组织中的表达情况，并分析其与肝细胞生长因子免疫反应及微血管数量的关系。结果：I-II期子宫内膜异位症患者在位内膜和异位内膜组织中MPHI的组织浸润率显著高于III-IV期子宫内膜异位症患者和对照组。与黑色或白色病变相比，红色腹膜病变及其邻近腹膜的Mphi浓度最高。这些炎细胞在月经周期的分泌期有较高的分布。在位内膜及相应红色病变的Mphi密度与HGF的Q-H评分及微血管密度均呈显著正相关。结论：子宫内膜异位症早期活动性腹膜病变及其邻近腹膜组织中含有丰富的肝细胞生长因子，可能参与了子宫内膜异位症的发生发展。
Objective: To investigate the distribution of macrophage (MO) infiltration in eutopic and ectopic endometrium, throughout the menstrual cycle.Design: Controlled clinical study using intact tissue.Setting: Nagasaki University School of Medicine, Nagasaki, Japan.Patient(s): Twenty infertile women with pelvic endometriosis and 20 women without endometriosis.Intervention(s): Biopsy specimens from peritoneal lesions and corresponding eutopic endometrium were collected from women with or without endometriosis. Adjacent peritoneal biopsies were also obtained from a fraction of these women. The activated Mphi marker CD68, mitogenic marker hepatocyte growth factor (HGF), and endothelial cell surface marker von Willebrand factor were immunolocalized and quantitated by light microscopy and Q-H score.Main Outcome Measure(s): Tissue infiltration of Mphi in eutopic endometrium, ectopic endometrium, and adjacent peritoneum was examined, and its relationship with the immunoreaction of HGF and microvessel number was analyzed. The possible production of HGF by the isolated basal Mphi was also examined.Result(s): Tissue infiltration of Mphi in the eutopic and ectopic endometrium, of women with stage I-II endometriosis was significantly higher than with stage III-IV endometriosis or in control women. Red peritoneal lesions and their adjacent peritoneum had the greatest Mphi concentration, compared with black or white lesions. These inflammatory cells showed a higher distribution in the secretory phase of the menstrual cycle. The Mphi density in the eutopic endometrium and corresponding red lesions showed a significant correlation with both Q-H score of HGF and microvessel density. A substantial amount of HGF was also produced by the isolated basal Mphi from women with endometriosis.Conclusion(s): These results suggest that the peritoneal lesions of early and active endometriosis and their adjacent peritoneum harbor abundant Mphi that could be involved in the growth of endometriosis.