Selective cell targeting with light-absorbing microparticles and nanoparticles
Selective cell targeting with light-absorbing microparticles and nanoparticles
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DOI：
10.1016/s0006-3495(03)75128-5
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发表时间：
2003-06-01
影响因子：
3.4
通讯作者：
Lin, CP
中科院分区：
文献类型：
--
作者：
Pitsillides, CM;Joe, EK;Lin, CP
我们描述了一种新的选择性细胞靶向方法，该方法基于使用由短激光脉冲加热的光吸收微粒和纳米颗粒来产生高度局部化的细胞损伤。该方法与发色团辅助的激光灭活和光动力学疗法密切相关，但仅由光吸收驱动，而不需要光化学中间体（特别是单线态氧）。光粒子相互作用的机制进行了研究，纳秒时间分辨显微镜和热模拟。光诱导损伤的程度进行了研究，细胞致死率，细胞膜通透性，和蛋白质失活。强烈的颗粒尺寸依赖性，发现这些相互作用。一种基于光靶向内源性颗粒的技术已经被用于治疗皮肤科和眼科的色素细胞。对于外源性颗粒，在该方法可以针对光动力疗法用于癌症治疗进行评估之前，需要进行药物代谢和生物分布研究。然而，与光敏剂不同，颗粒是独特的，因为它们可以在细胞中长时间保持稳定和惰性。因此，他们可能是特别有用的预标记细胞在工程组织植入前。随后用激光脉冲照射将允许以非侵入性方式控制植入的细胞（失活或调节）。
We describe a new method for selective cell targeting based on the use of light-absorbing microparticles and nanoparticles that are heated by short laser pulses to create highly localized cell damage. The method is closely related to chromophore-assisted laser inactivation and photodynamic therapy, but is driven solely by light absorption, without the need for photochemical intermediates (particularly singlet oxygen). The mechanism of light-particle interaction was investigated by nanosecond time-resolved microscopy and by thermal modeling. The extent of light-induced damage was investigated by cell lethality, by cell membrane permeability, and by protein inactivation. Strong particle size dependence was found for these interactions. A technique based on light to target endogenous particles is already being exploited to treat pigmented cells in dermatology and ophthalmology. With exogenous particles, phamacokinetics and biodistribution studies are needed before the method can be evaluated against photodynamic therapy for cancer treatment. However, particles are unique, unlike photosensitizers, in that they can remain stable and inert in cells for extended periods. Thus they may be particularly useful for prelabeling cells in engineered tissue before implantation. Subsequent irradiation with laser pulses will allow control of the implanted cells (inactivation or modulation) in a noninvasive manner.