The small auxin-up RNA OsSAUR45 affects auxin synthesis and transport in rice
The small auxin-up RNA OsSAUR45 affects auxin synthesis and transport in rice
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生长素上调小 RNA OsSAUR45 影响水稻生长素合成和运输
DOI：
10.1007/s11103-017-0595-7
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发表时间：
2017-05-01
影响因子：
5.1
通讯作者：
Jiang, De-An
中科院分区：
文献类型：
--
作者：
Xu, Yan-Xia;Xiao, Meng-Zhu;Jiang, De-An
关键信息本研究首次证明 OsSAUR45 通过抑制水稻中的 OsYUCCA 和 OsPIN 基因表达来影响生长素合成和运输，从而参与植物生长。 摘要生长素小 RNA (SAUR) 包含一个大的多基因家族，并作为植物中主要生长素反应的一部分被快速激活。然而，人们对 SAUR 在植物生长和发育中的作用知之甚少，尤其是在单子叶植物中。在这里，我们报告了OsSAUR45在模式植物水稻（Oryza sativa）中的生物学功能。OsSAUR45以组织特异性模式表达并且定位于细胞质。过度表达 OsSAUR45 的水稻品系表现出多效性发育缺陷，包括株高和初生根长度降低、不定根减少、叶片变窄和结实率降低。在 OsSAUR45 过表达系中，生长素水平和运输降低，可能是由于黄素结合单加氧酶家族蛋白 (OsYUCCA) 和 PIN 形成家族蛋白 (OsPIN) 的表达降低。外源生长素的应用快速诱导OsSAUR45表达并部分恢复过表达OsSAUR45的水稻品系的表型。这些结果表明OsSAUR45通过抑制OsYUCCA和OsPIN基因在水稻中的表达来影响植物生长素的合成和运输，从而参与植物生长。
Key messageThis research is the first to demonstrate that OsSAUR45 is involved in plant growth though affecting auxin synthesis and transport by repressing OsYUCCA and OsPIN gene expression in rice.AbstractSmall auxin-up RNAs (SAURs) comprise a large multigene family and are rapidly activated as part of the primary auxin response in plants. However, little is known about the role of SAURs in plant growth and development, especially in monocots. Here, we report the biological function ofOsSAUR45in the model plant rice (Oryza sativa).OsSAUR45is expressed in a tissue-specific pattern and is localized to the cytoplasm. Rice lines overexpressingOsSAUR45displayed pleiotropic developmental defects including reduced plant height and primary root length, fewer adventitious roots, narrower leaves, and reduced seed setting. Auxin levels and transport were reduced in theOsSAUR45overexpression lines, potentially because of decreased expression of Flavin-binding monooxygenase family proteins (OsYUCCAs) and PIN-FORMED family proteins (OsPINs). Exogenous auxin application rapidly inducedOsSAUR45expression and partially restored the phenotype of rice lines overexpressingOsSAUR45. These results demonstrate thatOsSAUR45is involved in plant growth by affecting auxin synthesis and transport through the repression ofOsYUCCAandOsPINgene expression in rice.