Cyclic AMP Level of Human Thyroid Cells in Monolayer Culture TSH Induced Refractoriness to TSH Action
Cyclic AMP Level of Human Thyroid Cells in Monolayer Culture TSH Induced Refractoriness to TSH Action
复制标题
单层培养 TSH 中人甲状腺细胞的环 AMP 水平诱导 TSH 作用无效
DOI：
10.1055/s-0028-1093660
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发表时间：
1976
影响因子：
2.2
通讯作者：
Y. Kaneko
中科院分区：
文献类型：
--
作者：
Y. Kaneko
来自患有格雷夫斯病的甲状腺功能正常患者的甲状腺细胞在化学成分确定的培养基中培养。当环磷酸腺苷浓度增加 8 倍时，细胞保留了对 TSH 做出反应的能力。这种环磷酸腺苷对 TSH 的反应因细胞事先暴露于 TSH 而减弱。 TSH 诱导的环 AMP 对 TSH 反应的降低是可逆的，与环 AMP 对 PGE1 反应的类似降低无关，并且不能归因于磷酸二酯酶活性增加或腺苷酸环化酶活性降低。先前暴露于TSH的甲状腺细胞对TSH刺激的部分抵抗可能是由于TSH受体的变化，这可能是由TSH本身引起的。
Thyroid cells from euthyroid patients with Graves' disease were cultured in a chemically defined medium. The cells preserved the ability to respond to TSH with 8-fold increase in cyclic AMP concentration. This cyclic AMP response to TSH was diminished by prior exposure of cells to TSH. The decrease in cyclic AMP response to TSH induced to TSH was reversible, was not associated with a similar decrease to cyclic AMP response to PGE1, and could not be attributed to increased phosphodiesterase activity or to decreased adenyl cyclase activity. The partial resistence to TSH stimulation of thyroid cells previously exposed to TSH may be due to changes in the TSH receptor, possibly caused by TSH itself.