pannier and pointedP2 act sequentially to regulate Drosophila heart development
pannier and pointedP2 act sequentially to regulate Drosophila heart development
复制标题
DOI：
10.1242/dev.00488
复制
发表时间：
2003-07-01
期刊：
影响因子：
4.6
通讯作者：
Skeath, JB
中科院分区：
文献类型：
--
作者：
Alvarez, AD;Shi, WY;Skeath, JB
果蝇的心脏由两种主要的细胞类型组成：成心细胞，形成心脏的收缩管；心包细胞位于成心细胞的旁边，被认为可以过滤和解毒苍蝇的血液或血淋巴。我们展示了所有心脏细胞的完整细胞系。值得注意的是，我们发现位于后七节段的心脏细胞谱系与位于更前面的心脏细胞谱系之间存在以前未被认识到的区别。通过基因筛选，我们发现ets转录因子是心脏后7节段成心细胞和心包细胞命运的关键调节因子。在这一领域，尖点促进心包细胞的发育，反对成心细胞的发育。我们发现，point的这种功能主要（如果不是完全）是由pointedP2异构体完成的，在这种情况下，pointedP2可能独立于Ras/MAPK途径的活性。我们继续证明GATA转录因子pannier在背中胚层发育早期起作用，促进心脏中胚层的发育，从而促进所有心脏细胞的发育。最后，我们证明pannier在pannier促进心脏中胚层形成的发育途径中起上游作用，而point随后在该区域起作用，以区分成心细胞和心包细胞的命运。
The Drosophila heart consists of two major cell types: cardioblasts, which form the contractile tube of the heart; and pericardial cells, which flank the cardioblasts and are thought to filter and detoxify the blood or hemolymph of the fly. We present the completion of the entire cell lineage of all heart cells. Notably, we detect a previously unappreciated distinction between the lineages of heart cells located in the posterior seven segments relative to those located more anteriorly. Using a genetic screen, we have identified the ETS-transcription factor pointed as a key regulator of cardioblast and pericardial cell fates in the posterior seven segments of the heart. In this domain, pointed promotes pericardial cell development and opposes cardioblast development. We find that this function of pointed is carried out primarily if not exclusively by the pointedP2 isoform and, that in this context, pointedP2 may act independently of Ras/MAPK pathway activity. We go on to show that the GATA transcription factor pannier acts early in dorsal mesoderm development to promote the development of the cardiac mesoderm and thus all heart cells. Finally, we demonstrate that pannier acts upstream of pointed in a developmental pathway in which pannier promotes cardiac mesoderm formation, and pointed acts subsequently in this domain to distinguish between cardioblast and pericardial cell fates.