Aurora A, mitotic entry, and spindle bipolarity
Aurora A, mitotic entry, and spindle bipolarity
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DOI：
10.1073/pnas.0601425103
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发表时间：
2006-04-11
影响因子：
11.1
通讯作者：
Ruderman, JV
中科院分区：
文献类型：
--
作者：
Liu, Q;Ruderman, JV
激酶Aurora-A（Aur-A），这是在中心体富集，是中心体成熟和准确的染色体分离所必需的，最近的工作暗示中心体作为最早的激活细胞周期蛋白B1-cdc 2发生的网站。在这里，我们使用非洲爪蟾卵提取物调查Aur-A的贡献，细胞周期的进展和纺锤体形态的存在或不存在的中心体。我们发现，除了积极的Aur-A加速cdc 2激活和有丝分裂进入。内源性Aur-A的消耗或非活性Aur-A的添加导致单极纺锤体，延迟但不阻断有丝分裂进入。这些对时间和纺锤体结构的影响并不需要中心体或染色体的存在。Aur-A的催化结构域本身足以恢复纺锤体双极性;额外的N-末端序列在有丝分裂定时中起作用。
The kinase Aurora-A (Aur-A), which is enriched at centrosomes, is required for centrosome maturation and accurate chromosome segregation, and recent work implicates centrosomes as sites where the earliest activation of cyclin B1-cdc2 occurs. Here, we have used Xenopus egg extracts to investigate Aur-A's contribution to cell cycle progression and spindle morphology in the presence or absence of centrosomes. We find that addition of active Aur-A accelerates cdc2 activation and mitotic entry. Depletion of endogenous Aur-A or addition of inactive Aur-A, which lead to monopolar spindles, delays but does not block mitotic entry. These effects on timing and spindle structure do not require the presence of centrosomes or chromosomes. The catalytic domain alone of Aur-A is sufficient to restore spindle bipolarity; additional N-terminal sequences function in mitotic timing.